The safety of meat from a microbiological standpoint is of paramount concern to public health, given the potential for bacterial contaminants to grow and persist during processing and storage. To address this issue, a culture-independent approach targeting the V3-V4 region of the 16S rRNA gene was utilized to investigate the inherent bacterial communities present in 10 chicken meat samples obtained from retail markets. Amplicons were sequenced using the Illumina MiSeq platform, and unique amplicon sequence variants (ASVs) were identified using the DADA2 pipeline. Results indicated the presence of 5 phyla, 7 classes, 16 orders, 33 families, 59 genera, and 273 unique ASVs. The dominant families were Flavobacteriaceae, Moraxellaceae, Enterobacteriaceae, Wohlfahrtiimonadaceae, Morganellaceae, and Pseudomonadaceae, comprising 27.03, 22.04, 15.67, 9.40, 7.92, and 5.02% of the identified families, respectively. Functional analysis using PICRUSt showed a diverse range of functional pathways. These findings have significant implications for policymaking regarding food safety and public health. Regular monitoring of bacterial communities in meat products is crucial to ensure their safety for consumption. This study demonstrates the utility of culture-independent approaches in characterizing microbial communities, which can provide valuable information for ensuring food safety and safeguarding public health.

从微生物角度来看，肉类的安全性对于公众健康至关重要，因为细菌污染物在加工和储存过程中可能会生长和持续存在。为了解决这个问题，利用一种针对16S rRNA基因V3-V4 区域的独立培养方法来研究从零售市场获得的 10 个鸡肉样品中存在的固有细菌群落。使用 Illumina MiSeq 平台对扩增子进行测序，并使用 DADA2 流程识别独特的扩增子序列变体 (ASV)。结果表明存在 5 个门、7 个纲、16 个目、33 个科、59 个属和 273 个独特的 ASV。优势科为黄杆菌科、莫拉菌科、肠杆菌科、沃尔法赫蒂莫纳达科、摩根菌科和假单胞菌科，分别占已鉴定科的27.03%、22.04%、15.67%、9.40%、7.92%和5.02%。使用 PICRUSt 进行的功能分析显示了多种功能途径。这些发现对食品安全和公共卫生的政策制定具有重大影响。定期监测肉制品中的细菌群落对于确保其消费安全至关重要。这项研究证明了独立于培养的方法在表征微生物群落方面的实用性，这可以为确保食品安全和维护公众健康提供有价值的信息。