Cases of diabetes are significantly increasing year by year, attracting the attention of medical professionals and researchers to focus on diabetes and its underlying complications. One among such are diabetic wounds which are difficult to heal, creating severe implications in the day-to-day chores of not only patients, but also family members. Dehydrozingerone (DHZ) is known to possess various effects like anti-inflammatory, anti-microbial, antioxidant, and wound-healing properties. The effect of DHZ on different phases of diabetic wound healing remains untested. Hence, this study was proposed to find out the effect of oral and topical formulation of DHZ on day 5, 10 and 15 of diabetic wound healing. Excisional wounds were created on the dorsal side of animals using punch biopsy to mimic human diabetic wounds. Topical DHZ gel (100 mg in 1 gm of gel) was prepared using 1% Carbopol 934 and was applied twice a day. The treated groups had increased percentage of wound closure; western blotting suggested that DHZ significantly increased ERK and JNK levels and decreased TNF and MMP 2 and 9 levels. From histopathological studies, it was observed that angiogenesis, collagen formation, granulation tissue formation, and fibroblast proliferation were improved on days 5, 10, and 15 of diabetic wound healing. These findings indicate that DHZ (both systemic and topical) are effective during the early phases of wound healing which gets impaired in diabetic wounds. Dehydrozingerone accelerated diabetic wound healing by regulating the various hallmarks of wound healing process.

摘要

糖尿病病例逐年显着增加，引起医学专业人士和研究人员对糖尿病及其潜在并发症的关注。其中之一是糖尿病伤口难以愈合，不仅对患者而且对家庭成员的日常琐事造成严重影响。众所周知，脱氢姜酮 (DHZ) 具有多种功效，如抗炎、抗菌、抗氧化和伤口愈合特性。 DHZ 对糖尿病伤口愈合不同阶段的影响尚未经过测试。因此，本研究旨在找出口服和外用 DHZ 制剂对糖尿病伤口愈合第 5、10 和 15 天的影响。使用穿刺活检在动物背侧制造切除伤口，以模仿人类糖尿病伤口。使用 1% Carbopol 934 制备局部 DHZ 凝胶（100 毫克溶于 1 克凝胶），每天涂抹两次。治疗组的伤口闭合百分比有所增加；蛋白质印迹表明 DHZ 显着增加 ERK 和 JNK 水平，并降低 TNF 和 MMP 2 和 9 水平。从组织病理学研究中观察到，糖尿病伤口愈合第5、10和15天时，血管生成、胶原蛋白形成、肉芽组织形成和成纤维细胞增殖得到改善。这些发现表明 DHZ（全身和局部）在伤口愈合的早期阶段是有效的，而糖尿病伤口会受到损害。脱氢姜油酮通过调节伤口愈合过程的各种特征来加速糖尿病伤口愈合。