Abstract

The possibility of active laser delivery of a modern chlorine-containing photosensitizing drug Chloderm (Chloderm, Russia) under the nail plate by laser radiation with a wavelength of 450 nm for the purpose of photodynamic therapy of onychomycosis is studied. In an in vitro experiment, sequential laser microporation of the nail plate and active delivery of the drug under the nail plate by this laser radiation with an intensity of up to 200 W/cm² were investigated. The results of studying the absorption spectra of an aqueous solution of Chloderm in the range of 400–900 nm before and after exposure to 450 nm laser radiation are presented. It is demonstrated that sequential laser microporation and active laser drug delivery under the nail plate is possible at laser radiation intensity greater than 178 W/cm². It is shown that the maximum rate and efficiency of nail plate ablation by 450 nm laser radiation is achieved at an intensity of 200 W/cm² and is 2750 ± 30 μm/s and 1.47 ± 0.05 μm/mJ, respectively. The delivery rate of Chloderm under the nail plate is 1.15 ± 0.1 mg/s. It is shown that exposure to 450 nm laser radiation at the intensity of 200 W/cm² for a time sufficient to deliver the drug under the nail plate does not change the extinction coefficient of the drug at the laser wavelength and slightly changes the conformational state of Chloderm.

中文翻译：

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波长 450 nm 激光辐射下治疗甲真菌病的现代含氯药物的激光传输和光谱研究

摘要

研究了通过波长 450 nm 的激光辐射将现代含氯光敏药物 Chloderm（Chloderm，俄罗斯）主动激光递送到指甲板下，用于甲癣光动力治疗的可能性。在体外实验中，研究了甲板的连续激光微孔化以及通过强度高达200 W/cm ²的激光辐射在甲板下主动递送药物。展示了 Chloderm 水溶液在暴露于 450 nm 激光辐射之前和之后在 400–900 nm 范围内的吸收光谱的研究结果。结果表明，在激光辐射强度大于178 W/cm ²时，连续激光微孔化和主动激光药物递送在甲板下是可能的。结果表明，450 nm 激光辐射的甲板消融最大速率和效率在强度为 200 W/cm ²时实现，分别为 2750 ± 30 μm/s 和 1.47 ± 0.05 μm/mJ。 Chloderm 指甲板下的输送速率为 1.15 ± 0.1 mg/s。结果表明，暴露于强度为 200 W/cm ²的 450 nm 激光辐射足够长的时间以将药物递送到甲板下时，不会改变药物在激光波长下的消光系数，并且会轻微改变构象状态Chloderm。