We obtained the full-length cDNA clone of KAP26.1 in Liaoning cashmere goat, and then we further investigated biological functions of KAP26.1. First, we discovered KAP26.1 was specifically expressed in internal root sheath of skin hair follicles by semi-quantitative reverse transcription and polymerase chain reaction (semi-quantitative RT-PCR), hybridization in situ; immunohistochemistry revealed KAP26.1 was located in the internal and external root sheaths. Next, quantitative real-time polymerase chain reaction (qRT-PCR) results showed relative KAP26.1 expression quantity was significantly different between primary and secondary follicles during anagen, catagen, and remarkably increased during telogen. Moreover, after inhibiting Noggin expression, we found relative KAP26.1 expression quantity significantly declined; after KAP26.1 overexpression, we found relative Noggin expression quantity highly significantly declined. Finally, we found MT played a positive role in KAP26.1 and KAP26.1 expression; FGF5 and IGF-1 palyed a negative role in KAP26.1 and blocked the degradation of KAP26.1. The results revealed KAP26.1 played an important role in regulating fine hair development.

我们获得了辽宁绒山羊KAP26.1的全长cDNA克隆，并进一步研究了KAP26.1的生物学功能。首先，我们通过半定量逆转录和聚合酶链反应（半定量RT-PCR）、原位杂交发现KAP26.1在皮肤毛囊内根鞘中特异性表达；免疫组织化学显示KAP26.1位于内部和外部根鞘中。接下来，定量实时聚合酶链反应（qRT-PCR）结果显示，在毛发生长初期、毛发生长中期，初级和次级卵泡的相对KAP26.1表达量显着不同，并且在毛发生长终期显着增加。而且，抑制Noggin表达后，我们发现KAP26.1的相对表达量显着下降；KAP26.1过表达后，我们发现相对Noggin表达量显着下降。最后我们发现MT对KAP26.1以及KAP26.1的表达起到了积极的作用； FGF5和IGF-1对KAP26.1发挥负作用并阻止KAP26.1的降解。结果显示KAP26.1在调节细毛发育中发挥重要作用。