Abstract—

The protein endophilin A, which in the mammalian genome is encoded by three genes, endophilin A1, A2, and A3, regulates the synaptic vesicle cycle during exo- and endocytosis, and it is present in the reserve pool of synaptic vesicles (SVs), where its function is unknown. In vitro experiments suggest that endophilin, via its SH3 domain interactions, incorporates several components into the protein liquid phase that organizes SVs in the reserve pool. We investigated the effect of deletion of the genes encoding endophilin and one of its binding partners, dynamin, on the organization of SVs in living synapses formed by cortical neurons in culture. Our experiments showed that deletion of endophilin genes does not change the density of SVs in the reserve pool. At the same time, the deletion of dynamin 1 and dynamin 3 genes leads to a significant increase in the vesicle density. We suggest that other SH3-domain-containing proteins, which are components of the protein liquid phase, complement the function of endophilin in the SV reserve pool.

中文翻译：

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缺乏蛋白质液相成分的神经末梢突触小泡储备库的组织

摘要-

哺乳动物基因组中的内亲蛋白 A 蛋白由内亲蛋白 A1、A2和A3三个基因编码，在外吞作用和内吞作用期间调节突触小泡周期，并且存在于突触小泡 (SV) 的储备池中。其功能未知。体外实验表明，内亲素通过其 SH3 结构域相互作用，将多种成分掺入蛋白质液相中，从而在储备库中组织 SV。我们研究了编码内亲素及其结合伙伴之一动力蛋白的基因缺失对培养的皮层神经元形成的活突触中 SV 组织的影响。我们的实验表明，删除内亲素基因不会改变储备库中SV的密度。同时，动力蛋白1和动力蛋白3基因的缺失导致囊泡密度显着增加。我们建议其他含有 SH3 结构域的蛋白质（蛋白质液相的组成部分）可以补充 SV 储备库中内亲素的功能。