Slow transit constipation (STC) is a refractory gastrointestinal disease, accounting for approximately 13 ∼ 37 % of chronic constipation. However, the molecular mechanism of STC remains poorly understood. Herein, this study aims to identify the key mRNAs and lncRNAs associated with STC. To this end, we performed high-throughput RNA sequencing to identify differentially expressed (DE) mRNAs and lncRNAs in the whole-layer sigmoid intestinal tissues from 4 STC patients and 4 non-STC patients. The identified DE lncRNAs and mRNAs were validated through quantitative real-time PCR. Weighted gene co-expression network analysis (WGCNA) and Pearson correlation analysis were conducted to determine the significantly correlated DE mRNA-lncRNA pairs. A total of 1420 DE lncRNAs and 1634 DE mRNAs were identified. Kyoto Encyclopedia of Genes and Genomes analysis of DE mRNAs indicated that these DE mRNAs might be associated with systemic lupus erythematosus, alcoholism, intestinal immune network for IgA production, inflammatory bowel disease, NF-kappa B signaling pathway. WGCNA and Pearson correlation analyses jointly identified 16,577 significantly correlated DE mRNA-lncRNA pairs. Furthermore, lncRNAs LINC00641, LINC02268, LINC03013 were identified as hub lncRNAs. The protein–protein interaction (PPI) network of proteins encoded by DE mRNAs was established, and PPI-based analysis revealed that Interleukin 2(IL2), CD80 molecule (CD80), interleukin-17A (IL-17A) might play significant roles in the development of STC. This study analyzes the expression profiles of lncRNAs and mRNAs associated with STC. Our findings will contribute to further understanding of the molecular mechanism of STC and provide potential diagnostic or therapeutic biomarkers for STC.

中文翻译：

---

与慢传输型便秘相关的差异表达 lncRNA 和 mRNA 分析

慢传输型便秘（STC）是一种难治性胃肠道疾病，约占慢性便秘的13%～37%。然而，STC 的分子机制仍知之甚少。本研究旨在鉴定与 STC 相关的关键 mRNA 和 lncRNA。为此，我们进行了高通量RNA测序，以鉴定4名STC患者和4名非STC患者的全层乙状结肠肠组织中的差异表达（DE）mRNA和lncRNA。通过定量实时 PCR 验证了鉴定的 DE lncRNA 和 mRNA。进行加权基因共表达网络分析（WGCNA）和Pearson相关分析以确定显着相关的DE mRNA-lncRNA对。总共鉴定出 1420 个 DE lncRNA 和 1634 个 DE mRNA。京都基因和基因组百科全书对DE mRNA的分析表明，这些DE mRNA可能与系统性红斑狼疮、酗酒、IgA产生的肠道免疫网络、炎症性肠病、NF-κB信号通路有关。 WGCNA 和 Pearson 相关分析联合鉴定了 16,577 个显着相关的 DE mRNA-lncRNA 对。此外，lncRNA LINC00641、LINC02268、LINC03013 被确定为枢纽 lncRNA。建立了由 DE mRNA 编码的蛋白质的蛋白质相互作用 (PPI) 网络，基于 PPI 的分析表明白细胞介素 2 (IL2)、CD80 分子 (CD80)、白细胞介素 17A (IL-17A) 可能在STC的发展。本研究分析了与 STC 相关的 lncRNA 和 mRNA 的表达谱。我们的研究结果将有助于进一步了解 STC 的分子机制，并为 STC 提供潜在的诊断或治疗生物标志物。