Recently, mounting evidence has highlighted the essential function of the C-terminal binding protein-1 divergent transcript (CTBP1-DT) in malignancies. However, its role in kidney renal clear cell carcinoma (KIRC) remains largely unknown. Our study aimed to identify the potential function of CTBP1-DT in KIRC. RT-qPCR, Kaplan-Meier survival analysis, Cox regression analysis, and nomogram analysis were utilized to determine the expression and effects of CTBP1-DT on survival. The subcellular localization of CTBP1-DT was determined using RNA fluorescence hybridization (FISH). To investigate the functions of CTBP1-DT in regulating KIRC cell proliferation, migration, invasion, lipid synthesis, and apoptosis, we conducted CCK8, EdU, Transwell, and Oil Red O staining and cell apoptosis staining assays. The relationships between CTBP1-DT and the tumor microenvironment were investigated with multiple bioinformatics analysis algorithms and databases, including CYBERSORT, TIMER2, Spearman correlation test, tumor mutation burden (TMB), microsatellite instability (MSI), and immunophenoscore (IPS). According to our results, CTBP1-DT is a lncRNA located in the nucleus that is significantly upregulated in KIRC and is correlated with better clinical outcomes. Downregulating CTBP1-DT inhibited cell viability, migration, invasion, and lipid synthesis but triggered cell apoptosis. Additionally, we explored the potential effect of CTBP1-DT in regulating immune cell infiltration in KIRC and other malignancies. Furthermore, CTBP1-DT could be used to predict the effectiveness of targeted drugs and immune checkpoint inhibitors. In conclusion, we identified CTBP1-DT as a potential immunological biomarker and discovered the potential role of CTBP1-DT in regulating lipid synthesis and apoptosis resistance.

中文翻译：

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确定 CTBP1-DT 作为一种免疫生物标志物，可促进 KIRC 中的脂质合成和细胞凋亡抵抗

最近，越来越多的证据强调了 C 端结合蛋白 1 趋异转录本 (CTBP1-DT) 在恶性肿瘤中的重要功能。然而，其在肾透明细胞癌（KIRC）中的作用仍然很大程度上未知。我们的研究旨在确定 CTBP1-DT 在 KIRC 中的潜在功能。利用RT-qPCR、Kaplan-Meier生存分析、Cox回归分析和列线图分析来确定CTBP1-DT的表达和对生存的影响。使用 RNA 荧光杂交 (FISH) 确定 CTBP1-DT 的亚细胞定位。为了研究 CTBP1-DT 在调节 KIRC 细胞增殖、迁移、侵袭、脂质合成和凋亡中的功能，我们进行了 CCK8、EdU、Transwell 和油红 O 染色和细胞凋亡染色测定。利用多种生物信息学分析算法和数据库研究CTBP1-DT与肿瘤微环境之间的关系，包括CYBERSORT、TIMER2、Spearman相关性检验、肿瘤突变负荷（TMB）、微卫星不稳定性（MSI）和免疫表型评分（IPS）。根据我们的结果，CTBP1-DT 是一种位于细胞核中的 lncRNA，在 KIRC 中显着上调，并且与更好的临床结果相关。下调 CTBP1-DT 可抑制细胞活力、迁移、侵袭和脂质合成，但会引发细胞凋亡。此外，我们还探讨了 CTBP1-DT 在调节 KIRC 和其他恶性肿瘤中免疫细胞浸润方面的潜在作用。此外，CTBP1-DT可用于预测靶向药物和免疫检查点抑制剂的有效性。总之，我们确定 CTBP1-DT 作为潜在的免疫生物标志物，并发现 CTBP1-DT 在调节脂质合成和细胞凋亡抵抗中的潜在作用。