It is generally accepted that jasmonate‐ZIM domain (JAZ) repressors act to mediate jasmonate (JA) signaling via CORONATINE‐INSENSITIVE1 (COI1)‐mediated degradation. Here, we report a cryptic signaling cascade where a JAZ repressor, FvJAZ12, mediates multiple signaling inputs via phosphorylation‐modulated subcellular translocation rather than the COI1‐mediated degradation mechanism in strawberry (Fragaria vesca). FvJAZ12 acts to regulate flavor metabolism and defense response, and was found to be the target of FvMPK6, a mitogen‐activated protein kinase that is capable of responding to multiple signal stimuli. FvMPK6 phosphorylates FvJAZ12 at the amino acid residues S179 and T183 adjacent to the PY residues, thereby attenuating its nuclear accumulation and relieving its repression for FvMYC2, which acts to control the expression of lipoxygenase 3 (FvLOX3), an important gene involved in JA biosynthesis and a diverse array of cellular metabolisms. Our data reveal a previously unreported mechanism for JA signaling and decipher a signaling cascade that links multiple signaling inputs with fruit trait development.

中文翻译：

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通过磷酸化调节 JAZ 阻遏物核积累介导果实性状发育的信号级联

人们普遍认为，茉莉酸-ZIM 结构域 (JAZ) 阻遏物通过 CORONATINE-INSENSITIVE1 (COI1) 介导的降解来介导茉莉酸 (JA) 信号传导。在这里，我们报告了一个神秘的信号级联，其中 JAZ 阻遏物 FvJAZ12 通过磷酸化调节的亚细胞易位而不是草莓中 COI1 介导的降解机制介导多个信号输入。草莓）。 FvJAZ12 可以调节风味代谢和防御反应，并被发现是 FvMPK6 的靶标，FvMPK6 是一种丝裂原激活蛋白激酶，能够响应多种信号刺激。 FvMPK6 在与 PY 残基相邻的氨基酸残基 S179 和 T183 处磷酸化 FvJAZ12，从而减弱其核积累并解除其对 FvMYC2 的抑制，FvMYC2 的作用是控制脂氧合酶 3 的表达。FvLOX3），一个参与 JA 生物合成和多种细胞代谢的重要基因。我们的数据揭示了之前未报道的 JA 信号传导机制，并破译了将多个信号输入与果实性状发育联系起来的信号级联。