Platycodi radix is a widely used herbal medicine that contains numerous phytochemicals beneficial to health. The health and biological benefits of P. radix have been found across various diseases. The utilization of umbilical cord stromal stem cells, derived from Wharton's jelly of the human umbilical cord, has emerged as a promising approach for treating degenerative diseases. Nevertheless, growing evidence indicates that the function of stem cells declines with age, thereby limiting their regenerative capacity. The primary objective in this study is to investigate the beneficial effects of P. radix in senescent stem cells. We conducted experiments to showcase that diminished levels of Lamin B1 and Sox‐2, along with an elevation in p21, which serve as indicative markers for the senescent stem cells. Our findings revealed the loss of Lamin B1 and Sox‐2, coupled with an increase in p21, in umbilical cord stromal stem cells subjected to a low‐dose (0.1 μM) doxorubicin (Dox) stimulation. However, P. radix restored the Dox‐damage in the umbilical cord stromal stem cells. P. radix reversed the senescent conditions when the umbilical cord stromal stem cells exposed to Dox‐induced reactive oxygen species (ROS) and mitochondrial membrane potential are significantly changed. In Dox‐challenged aged umbilical cord stromal stem cells, P. radix reduced senescence, increased longevity, prevented mitochondrial dysfunction and ROS and protected against senescence‐associated apoptosis. This study suggests that P. radix might be as a therapeutic and rescue agent for the aging effect in stem cells. Inhibition of cell death, mitochondrial dysfunction and aging‐associated ROS with P. radix provides additional insights into the underlying molecular mechanisms.

中文翻译：

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桔梗水提取物通过减少脐带基质干细胞中线粒体活性氧来挽救阿霉素诱导的衰老

桔梗是一种广泛使用的草药，含有多种对健康有益的植物化学物质。的健康和生物学益处根草已在多种疾病中被发现。利用源自人类脐带沃顿胶的脐带基质干细胞已成为治疗退行性疾病的一种有前景的方法。然而，越来越多的证据表明，干细胞的功能随着年龄的增长而下降，从而限制了它们的再生能力。本研究的主要目的是研究以下物质的有益影响：根草在衰老干细胞中。我们进行的实验表明 Lamin B1 和 Sox-2 水平降低，同时 p21 水平升高，这些可作为衰老干细胞的指示标记。我们的研究结果显示，在接受低剂量（0.1 μM）阿霉素（Dox）刺激的脐带基质干细胞中，Lamin B1 和 Sox-2 丢失，同时 p21 增加。然而，根草恢复了脐带基质干细胞中的 Dox 损伤。根草当脐带基质干细胞暴露于阿霉素诱导的活性氧（ROS）和线粒体膜电位显着改变时，逆转了衰老状况。在受到 Dox 攻击的老化脐带基质干细胞中，根草减少衰老，延长寿命，防止线粒体功能障碍和活性氧，并防止衰老相关的细胞凋亡。这项研究表明根草可能作为干细胞衰老效应的治疗和救援剂。抑制细胞死亡、线粒体功能障碍和衰老相关的 ROS根草提供了对潜在分子机制的更多见解。