Discharges to the aquatic environment of pharmaceuticals represent a hazard to the aquatic organisms. Subchronic assay with 17‐alpha‐ethinylestradiol (EE2) and in vitro essays with pharmaceuticals of environmental concern were conducted to examine the sensitivity of tissue acetylcholinesterase (AChE) and carboxylesterase (CbE) activities of Tinca tinca to them. Subchronic exposure to 17‐alpha‐EE2 caused significant effects on brain, liver, and muscle CbE, but no on AChE activities. Most of the pharmaceuticals tested in vitro were considered as weak inhibitors of tissular AChE activity. Depending on the tissues, some compounds were classified as moderate inhibitors of CbE activity while other were categorized as weak enzymatic inhibitors. An opposite trend was observed depending on the tissue, while brain and liver CbE activities were inhibited, the muscle CbE activity was induced. Changes experienced on enzymatic activities after exposure to pharmaceuticals might affect the physiological functions in which these enzymes are involved. In vitro exposure to 17‐alpha‐EE2 in tench could be an informative, but not a surrogate model to know the effect of this synthetic estrogen on AChE and CbE activities.

中文翻译：

---

Tinca Tinca 中胆碱酯酶和羧酸酯酶对药品的敏感性

药物排放到水生环境会对水生生物造成危害。 17-α-炔雌醇亚慢性测定（EE2） 和体外进行了有关环境问题的药物的论文，以检查组织乙酰胆碱酯酶（AChE）和羧酸酯酶（CbE）活性的敏感性丁卡丁卡给他们。亚慢性暴露于 17-α-EE2对大脑、肝脏和肌肉 CbE 产生显着影响，但对 AChE 活性没有影响。大多数药物都经过测试体外被认为是组织 AChE 活性的弱抑制剂。根据组织的不同，一些化合物被归类为 CbE 活性的中度抑制剂，而其他化合物则被归类为弱酶抑制剂。根据组织的不同，观察到相反的趋势，脑和肝脏的CbE活性受到抑制，而肌肉的CbE活性被诱导。接触药物后酶活性的变化可能会影响这些酶所涉及的生理功能。体外接触 17-α-EE2in tench 可能是一种信息丰富的模型，但不是了解这种合成雌激素对 AChE 和 CbE 活性影响的替代模型。