IntroductionGomisin is a natural dibenzo cyclooctene lignan, which is mainly derived from the family Magnoliaceae. It has anti‐inflammatory, antioxidant, anti‐tumor, anti‐aging, and hypoglycemic effects. Gomisins play important roles as medicines, nutraceuticals, food additives, and cosmetics.ObjectiveThe objective of this study is to establish a micellar electrokinetic chromatography (MEKC) method for simultaneous separation and determination of seven biphenyl cyclooctene lignans (Gomisin D, E, G, H, J, N, and O) in Schisandra chinensis and its preparations.MethodsThe method was optimized by studying the effects of the main parameters on the separation. The method has been validated and successfully applied to the determination of seven Gomisins in S. chinensis and its preparations.ResultsIn the separation system, the running buffer was composed of 20 mM Na2HPO4, 8.0 mM sodium dodecyl sulfate (SDS), 11% (v/v) methanol, and 6.0% (v/v) ethanol. A diode array detector was used with a detection wavelength of 230 nm, a separation voltage of 17 kV, and an operating temperature of 25°C. Under this condition, the seven analytes were separated at baseline within 20 min, and a good linear relationship was obtained with correlation coefficient ranging from 0.9919 to 0.9992. The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) ranged from 0.8 to 0.9 μg/mL and from 2.6 to 3.0 μg/mL, respectively. The recovery rate was between 99.1% and 102.5%.ConclusionThe experimental results indicated that this method is suitable for the separation and determination of seven Schisandra biphenyl cyclooctene lignan compounds in real samples. At the same time, it provides an effective reference for the quality control of S. chinensis and its preparations.

中文翻译：

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双有机溶剂体系胶束电动色谱同时分离测定五味子及其制剂中7种联苯环辛烯木脂素

简介Gomisin是一种天然二苯并环辛烯木脂素，主要来源于木兰科。它具有抗炎、抗氧化、抗肿瘤、抗衰老、降血糖等作用。 Gomisin在医药、保健品、食品添加剂、化妆品等领域发挥着重要作用。目的建立同时分离测定7种联苯环辛烯木脂素（Gomisin D、E、G、H）的胶束电动色谱（MEKC）方法。 、J、N 和 O) 中五味子方法通过研究主要参数对分离效果的影响，优化方法。该方法已被验证并成功应用于7种Gomisins的测定中华龙胆结果分离系统中，运行缓冲液由20 mM Na2羟基磷灰石4、8.0 mM 十二烷基硫酸钠 (SDS)、11% (v/v) 甲醇和 6.0% (v/v) 乙醇。采用二极管阵列检测器，检测波长为230 nm，分离电压为17 kV，工作温度为25℃。在此条件下，7种分析物在20 min内即可实现基线分离，且线性关系良好，相关系数在0.9919~0.​​9992之间。检测限（LOD，S/N = 3）和定量限（LOQ，S/N = 10）分别为0.8至0.9 μg/mL和2.6至3.0 μg/mL。回收率在99.1%~102.5%之间。结论实验结果表明该方法适用于七种物质的分离测定。五味子实际样品中的联苯环辛烯木脂素化合物。同时，为产品质量控制提供有效参考。中华龙胆及其制剂。