Fusarium wilt of lentil caused by Fusarium oxysporum f. sp. lentis (Fol) is a destructive pathogen limiting lentil production in India. In the present study, Secreted in Xylem (SIX) effectors genes were explored in Indian races of Fol and also a diagnostic tool for reliable detection of the disease was developed. Four SIX effectors genes, SIX11, SIX13, SIX6 and SIX2 were identified in 12 isolates of Fol belonging to seven races. SIX11 was present in all the races while SIX 13 was absent in race 6 and SIX6 was present only in race 4. The phylogenetic analysis revealed the conserved nature of the SIX genes within the forma specialis and showed sequence homology with F. oxysporum f. sp. pisi. The presence of three effectors, SIX11, SIX13 and SIX6 in race 4 correlates with high disease incidence in lentil germplasms. The in-silico characterization revealed the presence of signal peptide and localization of the effectors. Further SIX11 effector gene present in all the isolates was used to develop Fol-specific molecular marker for accurate detection. The marker developed could differentiate F. oxysporum f. sp. lycopersici, F. solani, F. oxysporum, Rhizoctonia solani and Sclerotium rolfsii and had a detection limit of 0.01ng μL^− 1. The effector-based marker detection helps in the unambiguous detection of the pathogen under field conditions.

扁豆枯萎病是由尖镰孢f.引起的。 sp。扁豆( Fol ) 是一种破坏性病原体，限制了印度扁豆的生产。在本研究中，在印度Fol种族中探索了木质部分泌( SIX ) 效应基因，并开发了可靠检测该疾病的诊断工具。在属于 7 个种族的12 个Fol分离株中鉴定出4 个SIX效应基因：SIX11、SIX13、SIX6和SIX2 。 SIX11存在于所有小种中，而SIX 13在小种 6 中不存在，而SIX6仅存在于小种 4 中。系统发育分析揭示了SIX基因在特制小种中的保守性，并显示出与F. oxysporum f的序列同源性。 sp。皮斯。种族4中三种效应子SIX11、SIX13和SIX6的存在与扁豆种质中的高发病率相关。计算机表征揭示了信号肽的存在和效应器的定位。进一步使用所有分离株中存在的SIX11效应基因来开发Fol特异性分子标记以进行准确检测。开发的标记可以区分F. oxysporum f. sp。番茄、茄镰刀菌、尖孢镰刀菌、立枯丝核菌和菌核菌，检测限为 0.01ng μL ^{- 1}。基于效应器的标记检测有助于在现场条件下明确检测病原体。