Abstract

Common horsetail (Equisetum arvense L.) is a rhizomatous and herbaceous perennial medicinal plant belonging to the Equisetaceae family, which is known as a rich source of phenolics and flavonoids. The previous studies have reported the anticancer, anti-inflammatory, antidiabetic, antioxidant, wound-healing, and hair- and nail-strengthening properties of the plant. The present study evaluated the effect of sonication time (6, 10, and 14 min), sonication temperature (20, 40, and 60 °C), and solvent concentration (0, 50, and 100% ethanol) on the total phenolic content (TPC), isoquercitroside amount, and antioxidant activity of the hydroalcoholic extract of E. arvense. In this regard, the shoots of E. arvense were extracted based on ultrasound-assisted hydroalcoholic extraction using response surface methodology (RSM). Additionally, the ferric-reducing antioxidant power (FRAP) assay was used to evaluate TPC, isoquercitroside quantity, and antioxidant activity under optimal conditions. The results suggested the sonication time of 6.91 min, sonication temperature of 27.88 °C, and solvent concentration of 56.62% ethanol (relative to water) as the best treatment. In general, the high R², adjusted R² (adj-R²), and P-values in the model fitting indicated the ideality of the delineated RSM models, as well as a suitable correlation between observed and predicted data.

中文翻译：

---

使用响应面法优化超声辅助从木贼中提取酚类物质及其抗氧化活性

摘要

木贼属（Equisetum arvense L.）是木贼科多年生根茎草本药用植物，以富含酚类和黄酮类化合物而闻名。之前的研究报道了该植物的抗癌、抗炎、抗糖尿病、抗氧化、伤口愈合以及强化头发和指甲的特性。本研究评估了超声处理时间（6、10 和 14 分钟）、超声处理温度（20、40 和 60 °C）以及溶剂浓度（0、50 和 100% 乙醇）对总酚含量的影响问荆水醇提取物的（TPC）、异槲皮苷含量和抗氧化活性。在这方面，使用响应面法（RSM）基于超声辅助水醇提取来提取问荆芽。此外，还使用铁还原抗氧化能力（FRAP）测定来评估最佳条件下的TPC、异槲皮苷含量和抗氧化活性。结果表明，超声处理时间为 6.91 分钟、超声处理温度为 27.88 °C、溶剂浓度为 56.62% 乙醇（相对于水）为最佳处理。一般来说，模型拟合中的高R ²、调整R ² (adj- R ² ) 和P值表明所描述的 RSM 模型的理想性，以及观测数据和预测数据之间的适当相关性。