Objectives: Dandelion has been shown to exert anti‐inflammatory and anti‐bacterial effects. Our study aimed to identify the effect and mechanism of dandelion flower extracts on H. pylori‐induced gastritis and screen for novel antimicrobial substances. Methods: Anti‐H. pylori activities of water extracts(WEDF) and ethanol extracts (EEDF) of dandelion flowers were performed with disk diffusion method assay, MIC, and MBC. The H. pylori‐induced model was constructed to examine the gastroprotective of EEDF using RUT, pathological analysis, and ELISA. Results: EEDF exhibited better anti‐ H. pylori and urease inhibition activities than WEDF. In vivo studies, EEDF can reduce the adhesion of H. pylori to the gastric mucosa, alleviate gastric damage, and concurrently reduce the levels of TNF‐α and IL‐6 in gastric tissues. The six phenolic compounds showed urease inhibition effect (IC50: 2.99 ± 0.15 to 66.08 ± 6.46 mmol/mL). Among them, chlorogenic acid, caffeic acid, and luteolin also had anti‐H. pylori activity (MIC: 64 ‐ 256 μg/mL). Conclusion: EEDF exhibited anti‐H. pylori, gastroprotective and anti‐inflammatory effects. Chicoric acid and luteolin may be the main active compounds of dandelion flowers to exert anti‐H. pylori, and worthy of further investigation.

中文翻译：

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蒲公英（Taraxacum mongolicum Hand.‐Mazz.）花提取物对抗幽门螺杆菌胃保护的潜力及其生物活性成分的表征

目的：蒲公英已被证明具有抗炎和抗菌作用。我们的研究旨在确定蒲公英花提取物对幽门螺杆菌引起的胃炎的作用和机制，并筛选新型抗菌物质。方法：抗H.采用纸片扩散法、MIC和MBC法检测蒲公英花水提取物(WEDF)和乙醇提取物(EEDF)的幽门螺杆菌活性。构建幽门螺杆菌诱导模型，利用 RUT、病理分析和 ELISA 检测 EEDF 的胃保护作用。结果：EEDF 比 WEDF 表现出更好的抗幽门螺杆菌和脲酶抑制活性。体内研究发现，EEDF可减少H. pylori对胃粘膜的粘附，减轻胃损伤，同时降低胃组织中TNF-α和IL-6的水平。六种酚类化合物均表现出脲酶抑制作用（IC50：2.99±0.15至66.08±6.46mmol/mL）。其中绿原酸、咖啡酸、木犀草素也具有抗H.幽门螺杆菌活性（MIC：64-256 μg/mL）。结论：EEDF 表现出抗 H.幽门螺杆菌、胃保护和抗炎作用。菊苣酸和木犀草素可能是蒲公英花抗幽门螺旋杆菌的主要活性化合物。 pylori，值得进一步研究。