This work explores the potential of porphyrin-grafted lignin nanoparticles (TCPP--AL-NPs) as a novel, effective, and biocompatible photosensitizer for antibacterial applications. Lignin acted as a sustainable framework, enhancing water-compatibility and single oxygen (O) generation of porphyrin. The mechanism study revealed the successful formation of a p-n heterojunction between lignin and porphyrin, which promoted the charge separation and the photocatalytic process of O to O. antibacterial assessments demonstrated the efficacy of TCPP--AL-NPs in eradicating both Gram-negative () and Gram-positive () bacteria under blue LED light irradiation. The observed lower antibacterial effectiveness against compared to could be attributed to the thicker cell membrane of Gram-negative bacteria. Additionally, TCPP--AL-NPs exhibited no cytotoxicity towards mouse fibroblast cells (L929). This study presents a straightforward and promising approach for developing lignin-based photosensitizers for photodynamic therapy.

中文翻译：

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卟啉共轭木质素 pn 异质结作为抗菌应用的有效且生物相容性光敏剂

这项工作探索了卟啉接枝木质素纳米粒子（TCPP--AL-NPs）作为一种新型、有效且生物相容性光敏剂用于抗菌应用的潜力。木质素充当可持续框架，增强水相容性和卟啉的单氧（O）生成。机理研究揭示了木质素和卟啉之间成功形成了 pn 异质结，促进了 O 到 O 的电荷分离和光催化过程。抗菌评估证明了 TCPP--AL-NPs 消除革兰氏阴性菌的功效（）蓝色LED灯照射下的革兰氏阳性（+）细菌。与相比，观察到的抗菌效果较低可能是由于革兰氏阴性菌的细胞膜较厚。此外，TCPP--AL-NPs 对小鼠成纤维细胞 (L929) 没有表现出细胞毒性。这项研究提出了一种直接且有前景的方法来开发用于光动力疗法的木质素光敏剂。