Bone defects induced by trauma, infection, and tumors present a significant challenge for treatment in clinical trials. Biomaterial-based tissue engineering strategy has shown great promise in bone defect repair. However, multiple functions inherent in the extracellular matrix (ECM) are rarely covered in the design of scaffold materials associated with bone grafting procedures. These deficiencies significantly limit its potential application in pathologic bone defect therapy. In this work, we constructed a hydrogel scaffold mimicking the dynamic features of ECM with the combination of antimicrobial and osteogenic capabilities as well, to investigate its applications in the therapy of infected radial defects. The hydrogel scaffold enables not only minimally invasive implantation but also adaptability and adhesion to irregular defect shapes. Both in vitro and in vivo assays have indicated that the hydrogel scaffold could rapidly eradicate bacteria, restore the infection-induced localized inflammatory microenvironment. Further loading osteogenic growth peptide (OGP) to recruit stem cells promotes osteogenic differentiation and ultimately enhances bone regeneration in the defect region. In summary, our work indicated that hydrogels with ECM-related inherent bioactivities and dynamic inner structures represent a feasible solution as a tissue bionic substitute for the therapy of infected bone defects.

中文翻译：

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基于抗生素识别的可注射成骨水凝胶用于感染桡骨骨缺损的再生

创伤、感染和肿瘤引起的骨缺损对临床试验的治疗提出了重大挑战。基于生物材料的组织工程策略在骨缺损修复方面显示出巨大的前景。然而，与骨移植手术相关的支架材料的设计很少涵盖细胞外基质（ECM）固有的多种功能。这些缺陷极大地限制了其在病理性骨缺损治疗中的潜在应用。在这项工作中，我们构建了一种模仿 ECM 动态特征并结合抗菌和成骨功能的水凝胶支架，以研究其在治疗感染性桡骨缺损中的应用。水凝胶支架不仅能够实现微创植入，而且能够对不规则缺损形状具有适应性和粘附性。体外和体内实验均表明水凝胶支架可以快速消灭细菌，恢复感染引起的局部炎症微环境。进一步加载成骨生长肽（OGP）来招募干细胞，促进成骨分化，最终增强缺损区域的骨再生。总之，我们的工作表明，具有 ECM 相关固有生物活性和动态内部结构的水凝胶代表了作为治疗感染性骨缺损的组织仿生替代品的可行解决方案。