Activity-based anorexia (ABA) is a rodent model of anorexia nervosa (AN) that induces several key components of AN, including voluntary reduction in food intake, reduced body weight, hyperactivity, and alterations to the hypothalamic-pituitary-adrenal (HPA) axis. Previous research has demonstrated persistently increased anxiety-like behavior in the elevated plus maze (EPM), a test measuring avoidance of novel and open areas in adult female rats that experienced ABA during adolescence and are weight-restored in adulthood. Whether the same behavioral effects of two bouts of adolescent ABA emerge in response to different anxiety-provoking stimuli, however, has not been explored. We used the social partition (SP), novelty suppressed feeding (NSF), marble burying, and EPM tests to explore whether two bouts of adolescent ABA have persistent effects on anxiety-like behavior in weight restored young adult female rats. One-way ANOVA analyses revealed that female rats that experienced two bouts of ABA during adolescence had increased anxiety-like behavior in the EPM and SP tests in young adulthood following weight restoration compared with controls. These data demonstrate that the enduring behavioral effects of two bouts of adolescent ABA are specific to particular anxiety-provoking stimuli and suggest that adolescent ABA has enduring effects on social relationships.

中文翻译：

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青少年活动性厌食症对年轻成年雌性大鼠的社会行为具有重大而长期的影响

基于活动的厌食症 (ABA) 是一种神经性厌食症 (AN) 的啮齿动物模型，可诱发 AN 的几个关键组成部分，包括自愿减少食物摄入、体重减轻、多动以及下丘脑 - 垂体 - 肾上腺 (HPA) 的改变轴。先前的研究表明，高架十字迷宫 (EPM) 中的焦虑样行为持续增加，EPM 是一项测量成年雌性大鼠对新奇和开放区域的回避程度的测试，这些大鼠在青春期经历 ABA，并在成年期体重恢复。然而，两次青春期 ABA 是否会因不同的焦虑刺激而出现相同的行为效应，这一点尚未被探索。我们使用社会分区（SP）、新奇抑制进食（NSF）、弹珠埋藏和 EPM 测试来探讨两次青春期 ABA 是否对体重恢复的年轻成年雌性大鼠的焦虑样行为有持续影响。单向方差分析显示，与对照组相比，在青春期经历过两次 ABA 的雌性大鼠在成年后体重恢复后的 EPM 和 SP 测试中焦虑样行为有所增加。这些数据表明，两次青少年 ABA 的持久行为影响是针对特定的引发焦虑的刺激，并表明青少年 ABA 对社会关系具有持久的影响。