Insufficient sleep adversely affects energy homeostasis by decreasing leptin levels. The underlying physiological mechanisms; however, remain unclear. Circulating leptin is well described to be regulated by its soluble receptor (sOB-R). Intriguingly, the impact of short sleep duration on sOB-R levels has never been characterized. In this study, we investigated, for the first time, the variation of sOB-R levels and its temporal relationship with circulating leptin upon acute sleep restriction. Five adult females were maintained on an 8-hour sleep schedule (bedtime at 00:00) for 1 week before restricting their sleep to 4.5 h (bedtime at 03:30) on 2 consecutive nights. Balanced meals were scheduled to specific hours and sleep was objectively measured. Four-hour blood samples were regularly collected during waking hours between 08:00 and 00:00. Sleep restriction resulted in lower leptin (20.9 ± 1.7 vs 25.7 ± 1.7 ng/ml) and higher sOB-R concentrations (24.4 ± 1.2 vs 19.8 ± 1.6 ng/ml). Neither the discordant temporal relationship nor the pattern of leptin and sOB-R were altered in response to sleep restriction. Our results suggest that sleep restriction may modulate circulating leptin levels and possibly metabolism via upregulating its soluble receptor. This observation may have valuable therapeutic implications when considering sOB-R as a potential target during the management of metabolic disturbances.

中文翻译：

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急性睡眠限制后循环瘦素受体水平的特征：针对健康成年女性的初步研究

睡眠不足会降低瘦素水平，从而对能量稳态产生不利影响。潜在的生理机制；然而，仍不清楚。循环瘦素被充分描述为受其可溶性受体 (sOB-R) 调节。有趣的是，短睡眠时间对 sOB-R 水平的影响从未被描述过。在这项研究中，我们首次研究了急性睡眠限制时 sOB-R 水平的变化及其与循环瘦素的时间关系。 5 名成年女性保持 8 小时睡眠计划（00:00 就寝）持续 1 周，然后连续 2 晚将睡眠时间限制在 4.5 小时（03:30 就寝）。均衡膳食被安排在特定的时间，并且客观地测量睡眠。在 08:00 至 00:00 清醒时间段定期采集四小时血样。睡眠限制导致瘦素降低（20.9 ± 1.7 vs 25.7 ± 1.7 ng/ml）和较高的 sOB-R 浓度（24.4 ± 1.2 vs 19.8 ± 1.6 ng/ml）。不一致的时间关系以及瘦素和 sOB-R 的模式都没有因睡眠限制而改变。我们的结果表明，睡眠限制可能通过上调其可溶性受体来调节循环瘦素水平，并可能调节代谢。当将 sOB-R 视为代谢紊乱管理过程中的潜在靶点时，这一观察结果可能具有有价值的治疗意义。