This study focuses on speciation of air borne microbes in an office building and health impacts of these microbes in terms of Quantitative Microbial Risk Assessment (QMRA), Hazard Quotient (HQ) and Hazard Index (HI). This is important in the wake of Sustainability Development Goal 3 (Public Health and well-being) specially when there is a lack of standards in terms of microbial air quality in office buildings where people spend 8–10 h indoors. Air borne bacterial and fungal species in different floors of the office building with varying occupancy and work profile were identified. The load of bacterial count on an average for the entire building was found to be 1917 CFU/m3. Overall, Gram-positive bacteria were predominant (>53%). Based on V3–V4 sequencing (causing UTIs) was the most abundant (93.7%) and other bacterial species found were (gut commensals), (causes bacillary dysentery), and (acne producing). Fungal load was 4000 CFU/m3. Based on ITS sequencing (45.6%) was the dominant fungus and other fungi found were , and , , and , sp., . The QMRA of gram-negative air borne bacteria showed a high disease burden, well over the WHO benchmark values even though Hazard Quotient (HQ) and Hazard Index (HI) was found to be negligible. These findings can contribute to the development of guidelines for seemingly safe workplaces that may harbour disease-causing microbes and calls for an immediate attention from policy makers as it is a major cause of concern for public health.

中文翻译：

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办公楼内空气微生物的公共卫生风险评估和形态

本研究重点关注办公楼中空气传播微生物的形态以及这些微生物在定量微生物风险评估 (QMRA)、危害商 (HQ) 和危害指数 (HI) 方面对健康的影响。这对于实现可持续发展目标 3（公共健康和福祉）非常重要，特别是当人们在室内度过 8-10 小时的办公楼内的微生物空气质量缺乏标准时。确定了办公楼不同楼层、不同占用情况和工作情况下空气传播的细菌和真菌种类。整个建筑的平均细菌计数负荷为 1917 CFU/m3。总体而言，革兰氏阳性菌占主导地位（>53%）。根据 V3-V4 测序，（引起尿路感染）最丰富（93.7%），发现的其他细菌种类有（肠道共生菌）、（引起细菌性痢疾）和（产生痤疮）。真菌负荷为 4000 CFU/m3。根据 ITS 测序（45.6%）是优势真菌，发现的其他真菌为 、 、 、 、 、 sp. 、 。革兰氏阴性空气传播细菌的 QMRA 显示出很高的疾病负担，远高于世界卫生组织的基准值，尽管危害商（HQ）和危害指数（HI）可以忽略不计。这些发现有助于制定看似安全的工作场所指南，这些工作场所可能含有致病微生物，并呼吁政策制定者立即予以关注，因为这是公共卫生问题的一个主要原因。