Abstract

Majun Nisyan (MJN) is a polyherbal formulation used in Unani medicine for its therapeutic efficacy against Nisyan (forgetfulness/dementia). In the current work, an effort was made to develop and validate a new high-performance thin-layer chromatography (HPTLC) method for the quantitative estimation of the marker compounds (α + β) boswellic acids, β-asarone, isoeugenol, 6-gingerol and piperine from the formulation of MJN. The method employed HPTLC plates precoated with silica gel 60 F₂₅₄ as the stationary phase. The solvent system comprised of toluene‒ethyl acetate‒chloroform‒acetic acid (8:2:5:0.1, V/V). This method yields compact spots for (α + β) boswellic acids (R_F 0.102 ± 0.01), β-asarone (R_F 0.982 ± 0.04), isoeugenol (R_F 0.850 ± 0.03), 6-gingerol (R_F 0.698 ± 0.03) and piperine (R_F 0.355 ± 0.01). A CAMAG TLC Scanner 4 was used to carry out the densitometric scanning at 254 nm. The International Council for Harmonisation’s Q2(R1) guideline was used to validate the HPTLC method. Each gram of the MJN formulation was found to contain 0.0248 ± 0.0053 mg (α + β) boswellic acids, 0.0520 ± 0.0069 mg β-asarone, 0.0487 ± 0.0041 mg isoeugenol, 0.0468 ± 0.0023 mg 6-gingerol and 0.0609 ± 0.0084 mg piperine. The developed and validated HPTLC method was found to be simple, inexpensive, sensitive, precise and accurate. This method can be easily applied to routine control analysis of all commercially available Ayurvedic and herbal formulations containing (α + β) boswellic acids, β-asarone, isoeugenol, 6-gingerol and piperine.

Graphical abstract

中文翻译：

---

高效薄层色谱法同时测定 Majun Nisyan 中的 (α + β) 乳香酸、β-细辛脑、异丁香酚、6-姜酚和胡椒碱

摘要

Majun Nisyan (MJN) 是 Unani 医学中使用的一种多草药制剂，具有针对Nisyan（健忘/痴呆）的治疗功效。在当前的工作中，我们努力开发和验证一种新的高效薄层色谱（HPTLC）方法，用于定量评估标记化合物（α + β）乳香酸、β-细辛脑、异丁香酚、6- MJN 配方中的姜辣素和胡椒碱。该方法采用预涂有硅胶 60 F ₂₅₄作为固定相的 HPTLC 板。溶剂体系由甲苯-乙酸​​乙酯-氯仿-乙酸（8:2:5:0.1，V/V）组成。该方法可产生 (α + β) 乳香酸 ( R _F 0.102 ± 0.01)、β-细辛脑 ( R _F 0.982 ± 0.04)、异丁子香酚 ( R _F 0.850 ± 0.03)、6-姜酚 ( R _F 0.698 ± 0.03) 的致密斑点）和胡椒碱（RF _0.355 ± 0.01）。使用 CAMAG TLC Scanner 4 在 254 nm 处进行光密度扫描。国际协调委员会的 Q2(R1) 指南用于验证 HPTLC 方法。每克 MJN 配方含有 0.0248 ± 0.0053 mg (α + β) 乳香酸、0.0520 ± 0.0069 mg β-细辛脑、0.0487 ± 0.0041 mg 异丁香酚、0.0468 ± 0.0023 mg 6-姜酚和 0.0609 ± 0.0084毫克胡椒碱。开发和验证的 HPTLC 方法简单、廉价、灵敏、精确且准确。该方法可以轻松应用于所有含有 (α + β) 乳香酸、β-细辛脑、异丁香酚、6-姜酚和胡椒碱的市售阿育吠陀和草药制剂的常规控制分析。

图形概要