Purpose

We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus, a nematode parasitising sheep.

Methods

The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus. The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC₅₀ and LC₉₀ to determine the synergistic effect.

Results

The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect.

Conclusion

The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response.

中文翻译：

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青蒿正己烷提取物与万寿菊乙酸乙酯提取物对捻转血矛线虫的协同增效作用

目的

我们分析了青蒿和万寿菊组合的活性提取物对捻转血矛线虫（一种寄生于羊的线虫）的可能的协同活性。

方法

这项工作是在H. contortus的卵和感染性幼虫 (L3) 上进行的。结果用SAS 9.1进行分析，应用ANOVA和Tukey检验，并且使用SAS 9.1的Proc Probit通过回归分析确定致死浓度(LC)值LC50和LC90。另外，用LC ₅₀和LC ₉₀计算致死浓度(LC)，以确定协同效应。

结果

结果表明，所研究的两种植物对线虫卵和 L3 幼虫及其组合均具有高效能。 50/50 的组合获得了最高的卵孵化抑制效果，10 mg/mL 的25% A. cina和 75% T. lucida获得了最好的幼虫死亡率。此外，这种组合显示出协同效应。

结论

这里研究的两种植物由于其高生物活性作用和协同反应，可以作为天然驱虫药替代品。