Leaf volatile oil compositions of five species viz., , , , and * were examined in this study by Gas Chromatography-Flame Ionisation Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC–MS) analysis and percentage volatile compositions were compared. Thirty-eight compounds were identified that account for 90 to 99 % of essential oil composition from species, respectively. GC–MS profiling of essential oils revealed that leaf oils were predominantly characterized by limonene ( to ) as one of the major constituents. Monoterpenoids ( to ) are the prominent class of constituents present in the volatile oils of four plants under study whereas in one species (), sesquiterpene hydrocarbons () were the dominant category. The data from GC analyses were subjected to correlation study to analyse the correlation between the volatile constituents among the selected species. These essential oils were initially evaluated for their antioxidant capacity and the leaf oils exhibited promising radical scavenging property with an average IC value of μg/mL. This study was further designed to evaluate antimicrobial efficacy of five leaf essential oils against various pathogenic bacterial and fungal strains Four examined essential oils gave a comparative antibacterial activity with a minimum inhibitory concentration (MIC) value against tested bacterial strains ranging from leaf oils were found to be more sensitive to with MIC values ranged between to mg/mL. These essential oils also exhibited strong inhibitory potential against tested fungal strains, and , with MIC ranging from to mg/mL. Out of the five essential oils screened in this study, four of them have shown significant antimicrobial and antioxidant activities which can be attributed to synergistic potential of major monoterpenoid constituents present in these oils viz., geranial, neral, citronellal, nerol, geraniol, geranyl formate rather than the sesquiterpene dominated essential oil. Based on the findings obtained and conclusions derived, it is evident that the selected leaf essential oils are promising natural agents to fight free radical damage and antibiotic-resistant microbes.

中文翻译：

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通过 GC 分析和相关矩阵对柑橘精油进行挥发性化学分析和区分；其体外自由基清除和杀菌功效评价

本研究通过气相色谱-火焰离子化检测器 (GC-FID) 和气相色谱-质谱 (GC-MS) 分析检查了 、 、 、 和 * 五个物种的叶挥发油成分，并比较了挥发性成分百分比。鉴定出 38 种化合物，分别占物种精油成分的 90% 至 99%。精油的 GC-MS 分析表明，叶油的主要特征是柠檬烯 (to ) 作为主要成分之一。单萜 ( 至 ) 是所研究的四种植物挥发油中存在的主要成分，而在一个物种 () 中，倍半萜烃 () 是主要类别。对GC分析的数据进行相关性研究，以分析所选物种之间的挥发性成分之间的相关性。初步评估了这些精油的抗氧化能力，叶油表现出良好的自由基清除性能，平均 IC 值为 μg/mL。本研究进一步旨在评估五种叶精油对各种致病细菌和真菌菌株的抗菌功效。四种检查的精油对测试的细菌菌株（从叶油到MIC 值范围在 mg/mL 之间更敏感。这些精油还对测试的真菌菌株表现出强大的抑制潜力，并且 MIC 范围为 1 至 mg/mL。在本研究筛选的五种精油中，其中四种显示出显着的抗菌和抗氧化活性，这可归因于这些油中存在的主要单萜成分（即香叶醛、橙花醛、香茅醛、橙花醇、香叶醇、香叶基）的协同潜力。甲酸盐而不是倍半萜烯为主的精油。根据所获得的发现和得出的结论，所选择的叶精油显然是对抗自由基损伤和抗生素抗性微生物的有前途的天然制剂。