Ultrasound extraction (UE) enhanced with deep eutectic solvent (DES) was used to extract Lentinus edodes polysaccharides. Box-Behnken design (BBD) was applied to investigate the influences of water content (10–90 %), solid-liquid solvent (1 : 10–1 : 50 g/mL), time (4–12 min), temperature (40–80 °C) and ultrasonic power (100–500 W) on the yield of Lentinus edodes polysaccharides. The optimal extraction conditions were ultrasonic power of 300 W, extraction time of 8 min, water content of 80 %, a solid-liquid ratio of 1 : 30 g/mL and a temperature of 60 °C, respectively. The highest extraction yield of Lentinus edodes polysaccharide was 10.17 % under optimal conditions. The results of FT-IR, SEM, and monosaccharide composition confirmed that the extracts possessed the characteristics of polysaccharides. In addition, the polysaccharides obtained with the UE enhanced with DES method exhibited higher antioxidant activities than the polysaccharides extracted with the UE method and HWE method. This extraction method can further expand the production efficiency and structural diversity of Lentinus edodes polysaccharides and meet the supply and demand relationship. It can be foreseen that this method can be applied to the extraction of more active substances.

中文翻译：

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低共熔溶剂超声强化香菇多糖提取及其结构表征和抗氧化活性

采用低共熔溶剂（DES）增强超声提取（UE）提取香菇多糖。采用 Box-Behnken 设计 (BBD) 来研究含水量 (10–90 %)、固液溶剂 (1 : 10–1 : 50 g/mL)、时间 (4–12 分钟)、温度 ( 40–80 °C）和超声波功率（100–500 W）对香菇多糖产量的影响。最佳提取条件为超声功率300 W、提取时间8 min、含水量80%、料液比1:30 g/mL、温度60 ℃。在最佳条件下香菇多糖的提取率最高为10.17%。 FT-IR、SEM和单糖组成结果证实提取物具有多糖的特性。此外，DES法增强UE得到的多糖比UE法和HWE法提取的多糖表现出更高的抗氧化活性。该提取方法可以进一步扩大香菇多糖的生产效率和结构多样性，满足供需关系。可以预见，该方法可应用于更多活性物质的提取。