The high demand for bone grafts has motivated the development of implants with excellent osteogenic activity, whereas the risk of implant-associated infection, particularly given the rise of antimicrobial resistance, has compelled the development of implants with innovative antimicrobial strategies in which a small amount of bactericidal agent can effectively kill a wide range of bacteria. To induce antibacterial property, the surface of Grade-5 bone plate titanium implants used in clinical applications was modified using direct current (DC) sputter coating followed by thermal annealing. The 15 nm silver film-coated implants were thermally annealed in the furnace for 15 min at 750 °C. The modified implant surface’s antibacterial efficacy against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Salmonella typhi, and Methicillin-resistant staphylococcus aureus bacteria has been assessed using a colony-forming assay. On the modified implant surface, the growth of E. coli and S. aureus bacteria is reduced by 99.72%, while highly drug-resistant bacteria are inhibited by 96.59%. The MTT assay was used to assess the cytotoxicity of the modified bone-implant surface against NIH3T3 mouse fibroblast cells. The modified bone-implant surface promoted fibroblast growth and demonstrated good cytocompatibility. Furthermore, the mechanical properties of the implant were not harmed by this novel surface modification method. This method is simple and provides new insight into surface modification of commercial metallic implants to have effective antibacterial properties against various classes of bacteria.

中文翻译：

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通过银薄膜的热去湿对商用骨板 (Ti6Al4V) 植入物进行表面改性，以提高抗菌性和细胞相容性

对骨移植的高需求推动了具有优异成骨活性的植入物的开发，而植入物相关感染的风险，特别是考虑到抗菌素耐药性的上升，迫使开发具有创新抗菌策略的植入物，其中少量杀菌剂能有效杀灭多种细菌。为了诱导抗菌性能，临床应用中使用的 5 级骨板钛种植体的表面采用直流 (DC) 溅射涂层进行改性，然后进行热退火。 15 nm 银膜涂层植入物在 750 °C 的炉中热退火 15 分钟。改良种植体表面的抗菌功效大肠杆菌（大肠杆菌),金黄色葡萄球菌（金黄色葡萄球菌),伤寒沙门氏菌， 和耐甲氧西林金黄色葡萄球菌细菌已使用菌落形成测定进行评估。在改性种植体表面上，生长大肠杆菌和金黄色葡萄球菌细菌减少99.72%，高耐药菌抑制96.59%。 MTT 测定用于评估修饰的骨植入物表面对 NIH3T3 小鼠成纤维细胞的细胞毒性。修饰的骨植入物表面促进成纤维细胞生长并表现出良好的细胞相容性。此外，这种新颖的表面改性方法并未损害植入物的机械性能。该方法简单，为商业金属植入物的表面改性提供了新的见解，使其对各类细菌具有有效的抗菌特性。