species are known for their medicinal properties. However, due to similarities between species, the accurate identification of different species can be cumbersome. The study of morphoanatomy can contribute to the authentication of the species by providing additional information and contributing to the species characterization. Therefore, this work presents the morphoanatomy of the leaves and stems of (Reissek) Biral analyzed by optical microscopy, scanning electron microscopy, and histochemical approaches. Through these methods, we were able to determine, morphologically, the analyzed species features large and woody trees; its leaves are simple, distichous, and entire; the leaf margins are fringed; the stipules are caducous, triangular, and rarely persistent; the apex can vary from acute to obtuse, and the leaf blade has no trichomes. The frontal view presents polygonal epidermal cells, anomocytic, anisocytic, and tetracytic stomata that are found on the abaxial surface and are positioned at the same level as the ordinary epidermal cells; the epidermis is uniseriate and covered with a thick cuticle; the mesophyll is dorsiventral; the midrib is biconvex, and below the epidermis is the angular collenchyma, with one collateral bundle in an open arc and two dorsal bundles; in the ground parenchyma, there is a small collateral vascular bundle, with fibers juxtaposed to the phloem. Histochemical reactions were positive for lipophilic, phenolic, and lignified compounds. Among the observed characteristics, the stomatal classification of the leaf and the prominence of the midrib (adaxial surface) in this study help to identify and may contribute to species taxonomy by comparing it with other species of the genus and quality control studies.

中文翻译：

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Monteverdia evonymoides (Reissek) Biral 的形态解剖学和组织化学研究

物种以其药用特性而闻名。然而，由于物种之间的相似性，不同物种的准确识别可能很麻烦。形态解剖学的研究可以通过提供额外的信息和有助于物种表征来促进物种的鉴定。因此，这项工作通过光学显微镜、扫描电子显微镜和组织化学方法对 (Reissek) Biral 的叶和茎进行了形态解剖学分析。通过这些方法，我们能够从形态上确定所分析的物种特征是大型木本树木；它的叶子简单，二列，完整；叶缘有流苏；托叶早落，三角形，很少宿存；先端可以从锐尖到钝角变化，叶片没有毛状体。正面观可见多边形表皮细胞、不等细胞、不等细胞和四细胞气孔，位于远轴面，与普通表皮细胞位于同一水平；表皮单列，覆盖有厚角质层；叶肉位于背腹；中脉双凸，表皮下方有角状厚角组织，有1个呈开放弧形的侧束和2个背侧束。在地薄壁组织中，有一个小的侧支维管束，其纤维与韧皮部并列。亲脂性、酚类和木质化化合物的组织化学反应呈阳性。在观察到的特征中，本研究中叶子的气孔分类和中脉（近轴表面）的突出有助于通过与该属的其他物种进行比较和质量控制研究来识别并可能有助于物种分类。