A biochip assay provides high-throughput and multiplexed analysis of biological samples, chemicals, and natural products. Pruritus is itchiness due to several causes, such as allergy, dry skin, pregnancy, liver disease, kidney disease, and thyroid disease. Treatment of pruritus is associated with reduced immunoglobulin (IgE) and FcεRI levels in sera. Treatment of pruritus include corticosteroid creams or ointment. However, they have side effects, so we need a safer treatment using natural products. Here, we developed an assay using protein chip technology to identify natural products with antipruritic activity that inhibit IgE-FcεRI binding. Of the 28 tested natural product extracts, Corni Fructus extract inhibited human IgE-FcεRI binding and also showed anti-histamine effects in MC/9 mast cells. These results suggest that this protein biochip assay system can be used to identify promising natural product extracts for the treatment of pruritus.

中文翻译：

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人类 IgE-FcεRI 芯片检测筛选天然产物的止痒活性

生物芯片检测可对生物样品、化学品和天然产物进行高通量和多重分析。瘙痒症是由多种原因引起的瘙痒，例如过敏、皮肤干燥、怀孕、肝脏疾病、肾脏疾病和甲状腺疾病。瘙痒的治疗与血清中免疫球蛋白 (IgE) 和 FcεRI 水平降低有关。瘙痒的治疗包括皮质类固醇霜或软膏。然而，它们有副作用，因此我们需要使用天然产品进行更安全的治疗。在这里，我们开发了一种使用蛋白质芯片技术的检测方法来鉴定具有抑制 IgE-FcεRI 结合的止痒活性的天然产物。在 28 种测试的天然产物提取物中，山茱萸提取物抑制人 IgE-FcεRI 结合，并在 MC/9 肥大细胞中显示出抗组胺作用。这些结果表明，这种蛋白质生物芯片测定系统可用于识别有前途的天然产物提取物，用于治疗瘙痒。