Nuts are a well-known cause of food allergy and, once this has been diagnosed, due to the likelihood of cross-sensitization to multiple tree nut allergens, their strict avoidance from the diet is advisable. In this context, we present electrochemical bioplatforms to detect traces of hazelnut and walnut in processed foods through the determination of their respective allergenic proteins Cor a 9 and Jug r 1 in a fast and sensitive assay. First, the evaluation of the single determination of both proteins was performed by building sandwich immunoconjugates on the surface of magnetic microbeads relying on specific antibodies unmodified or conjugated to horseradish peroxidase. Amperometric transduction was made upon trapping the magnetic bioconjugates on the surface of disposable carbon electrodes, using the hydroquinone/hydrogen peroxide system. The great analytical performance achieved with the individual platforms (detection limits of 0.12 and 0.56 ng mL for Jug r 1 and Cor a 9, respectively), led us to the individual and dual quantification of both proteins in raw dough and baked cookies incurred with ground nuts. The developed method allowed detecting baked cookies incurred with 0.0025% ground walnut and 0.00002% ground hazelnut with results comparable to those provided by ELISA techniques. The feasibility of performing the dual determination of both allergens in a single run was demonstrated.

中文翻译：

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加工食品中核桃和榛子过敏蛋白的电化学免疫传感

坚果是众所周知的食物过敏原因，一旦确诊，由于可能对多种坚果过敏原交叉过敏，建议严格避免饮食。在这种背景下，我们提出了电化学生物平台，通过快速、灵敏的测定法测定加工食品中榛子和核桃各自的过敏蛋白 Cor a 9 和 Jug r 1 来检测它们的痕迹。首先，依靠未修饰或与辣根过氧化物酶缀合的特异性抗体，通过在磁性微珠表面构建夹心免疫缀合物来对两种蛋白质的单一测定进行评估。使用氢醌/过氧化氢系统将磁性生物缀合物捕获在一次性碳电极的表面上进行电流转导。各个平台实现了出色的分析性能（Jug r 1 和 Cor a 9 的检测限分别为 0.12 ng mL 和 0.56 ng mL），使我们能够对生面团和烘焙饼干中的蛋白质进行单独和双重定量。坚果。所开发的方法可以检测含有 0.0025% 碎核桃和 0.00002% 碎榛子的烘焙饼干，其结果与 ELISA 技术提供的结果相当。证明了在单次运行中对两种过敏原进行双重测定的可行性。