Affinity photolabeling is a smart method to study noncovalent and transient interactions and provide a submolecular picture of the contacts between interacting partners. In this review, we will focus on the identification of peptide partners using photoaffinity labeling coupled to mass spectrometry in different contexts such as in vitro with a purified potential partner, in model systems such as model membranes, and with live cells using both targeted and nontargeted proteomics studies. Different biological partners will be described, among which glycoconjugates, oligonucleotides, peptides, proteins, and lipids, with the photoreactive label inserted either on the peptide of interest or on the potential partner. Particular attention will be paid to the observation and characterization of specific rearrangements following the photolabeling reaction, which can help characterize photoadducts and provide a better understanding of the interacting systems and environment.

中文翻译：

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光亲和标记与 MS 结合识别肽生物伙伴：二次反应，是好还是坏？

亲和光标记是一种研究非共价和瞬时相互作用并提供相互作用伙伴之间接触的亚分子图像的智能方法。在这篇综述中，我们将重点关注在不同环境下使用光亲和标记与质谱联用来鉴定肽伴侣，例如在体外使用纯化的潜在伴侣、在模型系统（例如模型膜）中以及使用靶向和非靶向的活细胞蛋白质组学研究。将描述不同的生物伙伴，其中包括糖缀合物、寡核苷酸、肽、蛋白质和脂质，并将光反应标记插入感兴趣的肽或潜在的伙伴上。将特别关注光标记反应后特定重排的观察和表征，这有助于表征光加合物并提供对相互作用系统和环境的更好理解。