In vivo monitoring of multiple pesticide contamination is of great significance for evaluating the health risks of different pesticides, agricultural production safety, and ecological and environmental assessment. Here, we report a hydrogel microneedle array coupled light-addressable photoelectrochemical sensor for tracking multiple pesticide uptake and elimination in living animals and plants, holding three prominent merits: i) enables in-situ detection of in vivo pesticides, avoiding cumbersome and complex sample transportation and handling processes; ii) allows repeated in vivo sampling of the same organism, improving tracking test controllability and accuracy; iii) avoids lethal sampling, providing a better understanding of the pesticides fate in living organisms. The coupled sensor is mechanically robust for withstanding more than 0.35 N per needle and highly swellable (800 %) for timely extraction of sufficient in vivo solution for analysis. For proof-of-concept, it achieves in-situ detection of atrazine, acetamiprid, and carbendazim efficiently and quantitatively in artificial agarose skin models, mouse skin interstitial fluids, and plant leaves with little inflammatory reaction. This simple, highly integrated, minimally invasive, and high-throughput in vivo monitoring method is ideal for future field environmental monitoring and plant and animal disease diagnosis.

中文翻译：

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可膨胀微针耦合光寻址光电化学传感器用于体内多种农药污染的原位跟踪

多种农药污染的体内监测对于评估不同农药的健康风险、农业生产安全以及生态环境评价具有重要意义。在这里，我们报告了一种水凝胶微针阵列耦合光可寻址光电化学传感器，用于跟踪活体动植物中多种农药的吸收和消除，具有三个突出优点：i）能够对体内农药进行原位检测，避免繁琐和复杂的样品运输和处理流程； ii) 允许对同一生物体进行重复体内采样，提高跟踪测试的可控性和准确性； iii) 避免致命取样，从而更好地了解农药在生物体中的归宿。耦合传感器机械坚固，每针可承受超过 0.35 N 的力，并且具有高度膨胀性 (800%)，可及时提取足够的体内溶液进行分析。为了进行概念验证，它在人造琼脂糖皮肤模型、小鼠皮肤间质液和植物叶片中实现了莠去津、啶虫脒和多菌灵的原位高效定量检测，且炎症反应极少。这种简单、高度集成、微创、高通量的体内监测方法非常适合未来的现场环境监测和动植物疾病诊断。