The development of high-performance sensors for doxycycline (DOX) detection is necessary because its residue accumulation will cause serious harm to human health and the environment. Here, a novel tri-emission ratiometric fluorescence sensor was proposed by using “post-mixing” strategy of different emissions fluorescence molecularly imprinted polymers with salicylamide as dummy template (DMIPs). BSA was chosen as assistant functional monomer, and also acted as sensitizers for the aggregation-induced emission (AIE) effect of DOX. The blue-emitting carbon dots and the red-emitting CdTe quantum dots were separately introduced into DMIPs as the response signals. Upon DOX recognition within 2 min, blue and red fluorescence of the tri-emission DMIPs sensor were quenched while green fluorescence of DOX was enhanced, resulting in a wide range of color variations observed over bluish violet-rosered-light pink-orange-yellow-green with a detection limit of 0.061 μM. The sensor possessed highly selective recognition and was successfully applied to detect DOX in complicated real samples. Moreover, with the fluorescent color collection and data processing, the smartphone-assisted visual detection of the sensors showed satisfied sensitivity with low detection limit. This work provides great potential applications for rapid and visual detection of antibiotics in complex substrates.

中文翻译：

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基于分子印迹的具有聚集诱导发射效应的三重发射比率荧光传感器，用于多西环素的视觉检测

开发用于强力霉素（DOX）检测的高性能传感器是必要的，因为其残留积累会对人类健康和环境造成严重危害。在这里，通过使用以水杨酰胺为虚拟模板（DMIP）的不同发射荧光分子印迹聚合物的“后混合”策略，提出了一种新型三发射比率荧光传感器。选择 BSA 作为辅助功能单体，同时也作为 DOX 聚集诱导发射 (AIE) 效应的敏化剂。将发蓝光的碳点和发红光的CdTe量子点分别引入DMIP中作为响应信号。在 2 分钟内识别 DOX 后，三发射 DMIPs 传感器的蓝色和红色荧光被猝灭，而 DOX 的绿色荧光增强，导致在蓝紫色-玫瑰红色-浅粉色-橙色-黄色-上观察到各种颜色变化。绿色，检测限为 0.061 μM。该传感器具有高选择性识别能力，并成功应用于复杂实际样品中的DOX检测。此外，通过荧光颜色采集和数据处理，智能手机辅助的传感器视觉检测显示出令人满意的灵敏度和较低的检测限。这项工作为快速、直观地检测复杂基质中的抗生素提供了巨大的潜在应用。