Abstract

The study aimed to elucidate the functional characteristics of OsASMT1 gene under copper (Cu) or sodium chloride (NaCl) stress. Bioinformatics scrutiny unveiled that OsASMT1 is situated on chromosome 9. Its protein architecture, comprising dimerization and methyltransferase domains, showed significant similarities to OsASMT2 and OsASMT3. High expression in roots and panicles, along with abiotic stress putative cis-regulatory elements in the promoter, indicated potential stress responsiveness. Real-time quantitative PCR confirmed OsASMT1 induction under Cu and NaCl stress in rice. Surprisingly, yeast expressing OsASMT1 did not exhibit enhanced resistance to abiotic stresses. The results of subcellular localization analysis indicated that OsASMT1 plays a role in the cytoplasm. While OsASMT1 responded to Cu and NaCl stress in rice, its heterologous expression in yeast failed to confer abiotic stress resistance, highlighting the need for further investigation of its functional implications.

中文翻译：

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OsASMT1 基因响应非生物胁迫的生物信息学和功能分析

摘要

该研究旨在阐明OsASMT1基因在铜（Cu）或氯化钠（NaCl）胁迫下的功能特征。生物信息学检查揭示了OsASMT1位于 9 号染色体上。其蛋白质结构（包括二聚化和甲基转移酶结构域）与OsASMT2和OsASMT3显着相似。根和圆锥花序中的高表达，以及启动子中非生物胁迫推定的顺式调节元件，表明潜在的胁迫反应性。实时定量 PCR 证实了水稻中 Cu 和 NaCl 胁迫下OsASMT1 的诱导。令人惊讶的是，表达OsASMT1的酵母并未表现出对非生物胁迫的增强抵抗力。亚细胞定位分析结果表明OsASMT1在细胞质中发挥作用。虽然OsASMT1对水稻中的铜和氯化钠胁迫作出反应，但其在酵母中的异源表达未能赋予非生物胁迫抗性，这突出表明需要进一步研究其功能意义。