Background: Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1 (NEDD1) serves as a crucial factor in promoting cellular mitosis by directly facilitating wheel assembly and daughter centriole biogenesis at the lateral site of parent centrioles, ultimately driving centrosome replication. The amplification of centrosomes and the abnormal expression of centrosome-associated proteins contribute to the invasion and metastasis of non-small cell lung cancer cells. However, the specific mechanism by which NEDD1 contributes to the progression of lung adenocarcinoma (LUAD) remains unexplored. Therefore, the objective of this study is to uncover the role played by NEDD1 in LUAD./nMethods: To verify the expression of NEDD1 in pan-carcinoma. The feasibility of NEDD1 as a prognostic marker for LUAD in TCGA and GEO databases was verified. Subsequently, Cox proportional hazard regression analysis was used to screen the prognostic factors of LUAD, so as to analyze the correlation between prognostic factors and NEDD1 expression. For another, NEDD1-related genes were screened for pathway enrichment analysis to verify their possible functions. In addition, the expression of NEDD1 in LUAD was verified by qPCR and IHC, then siRNA was used to construct NEDD1-knocked lung cancer cells for subsequent cytobehavioral experiments. Finally, the distribution of NEDD1 in single-cell samples was revealed, and then the correlation between its overexpression and LUAD immune escape and drug resistance was analyzed./nResults: LUAD exhibits upregulation of NEDD1, which in turn promotes the proliferation, migration, invasion, and epithelial-mesenchymal transition of lung cancer cells, thereby contributing to a poor prognosis. Furthermore, the overexpression of NEDD1 is closely associated with immune escape and drug resistance in LUAD./nConclusion: NEDD1 serves as a reliable prognostic marker for LUAD, and its upregulation is associated with increased immune escape and drug resistance. Given these findings, NEDD1 holds potential as a novel therapeutic target for the treatment of LUAD.

中文翻译：

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NEDD1 过表达会增加 LUAD 中的细胞增殖、肿瘤免疫逃逸和耐药性

背景：神经前体细胞表达的发育下调蛋白 1 (NEDD1) 通过直接促进母体中心粒侧面的轮组装和子中心粒生物发生，最终驱动中心体复制，从而成为促进细胞有丝分裂的关键因素。中心体的扩增和中心体相关蛋白的异常表达有助于非小细胞肺癌细胞的侵袭和转移。然而，NEDD1 促进肺腺癌 (LUAD) 进展的具体机制仍有待探索。因此，本研究的目的是揭示NEDD1在LUAD中所起的作用。/n方法：验证NEDD1在泛癌中的表达。在TCGA和GEO数据库中验证了NEDD1作为LUAD预后标志物的可行性。随后采用Cox比例风险回归分析筛选LUAD的预后因素，分析预后因素与NEDD1表达的相关性。另一方面，筛选NEDD1相关基因进行通路富集分析，以验证其可能的功能。此外，通过qPCR和IHC验证LUAD中NEDD1的表达，然后使用siRNA构建NEDD1敲除的肺癌细胞用于后续的细胞行为实验。最后揭示了NEDD1在单细胞样本中的分布，并分析了其过表达与LUAD免疫逃逸和耐药性之间的相关性。/n结果： LUAD表现出NEDD1的上调，进而促进增殖、迁移、肺癌细胞的侵袭和上皮间质转化，从而导致预后不良。此外，NEDD1 的过表达与 LUAD 的免疫逃逸和耐药性密切相关。/n结论： NEDD1 是 LUAD 的可靠预后标志物，其上调与免疫逃逸和耐药性增加相关。鉴于这些发现，NEDD1 有潜力成为治疗 LUAD 的新治疗靶点。