The ALOG (Arabidopsis LSH1 and Oryza G1) family proteins, namely, DUF640 domain-containing proteins, have been reported to function as transcription factors in various plants. However, the understanding of the response and function of ALOG family genes during reproductive development and under abiotic stress is still largely limited. In this study, we comprehensively analyzed the structural characteristics of ALOG family proteins and their expression profiles during inflorescence development and under abiotic stress in rice. The results showed that OsG1/OsG1L1/2/3/4/5/6/7/8/9 all had four conserved helical structures and an inserted Zinc-Ribbon (ZnR), the other four proteins OsG1L10/11/12/13 lacked complete Helix-1 and Helix-2. In the ALOG gene promoters, there were abundant cis-acting elements, including ABA, MeJA, and drought-responsive elements. Most ALOG genes show a decrease in expression levels within 24 h under ABA and drought treatments, while OsG1L2 expression levels show an upregulated trend under ABA and drought treatments. The expression analysis at different stages of inflorescence development indicated that OsG1L1/2/3/8/11 were mainly expressed in the P1 stage; in the P4 stage, OsG1/OsG1L4/5/9/12 had a higher expression level. These results lay a good foundation for further studying the expression of rice ALOG family genes under abiotic stresses, and provide important experimental support for their functional research.

中文翻译：

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水稻花序发育和非生物胁迫反应期间 ALOG 家族基因的表达谱（Oryza sativa L.）

ALOG（拟南芥LSH1 和稻G1)家族蛋白，即含有DUF640结构域的蛋白，已被报道在多种植物中充当转录因子。然而，对 ALOG 家族基因在生殖发育和非生物胁迫下的反应和功能的了解仍然很有限。本研究全面分析了水稻ALOG家族蛋白的结构特征及其在花序发育和非生物胁迫下的表达谱。结果显示，OsG1/OsG1L1/2/3/4/5/6/7/8/9均具有4个保守的螺旋结构和插入的锌带(ZnR)，其他4个蛋白OsG1L10/11/12/13缺乏完整的Helix-1和Helix-2。在ALOG基因启动子中，存在丰富的顺式-作用元件，包括ABA、MeJA和干旱响应元件。大多数 ALOG 基因在 ABA 和干旱处理下 24 小时内表现出表达水平下降，而骨G1L2ABA 和干旱处理下表达水平呈现上调趋势。花序发育不同阶段的表达分析表明骨G1L1/2/3/8/11主要表达于P1期；在P4阶段，骨细胞G1/骨G1L4/5/9/12具有较高的表达水平。这些结果为进一步研究水稻ALOG家族基因在非生物胁迫下的表达奠定了良好的基础，为其功能研究提供了重要的实验支持。