Posttranslational modification of histone proteins is critical for memory formation. Recently, we showed that monoubiquitination of histone H2B at lysine 120 (H2Bub) is critical for memory formation in the hippocampus. However, the transcriptome controlled by H2Bub remains unknown. Here, we found that fear conditioning in male rats increased or decreased the expression of 86 genes in the hippocampus but, surprisingly, siRNA-mediated knockdown of the H2Bub ligase, Rnf20, abolished changes in all but one of these genes. These findings suggest that monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation.

中文翻译：

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组蛋白 H2B 的单泛素化是记忆形成过程中转录组的重要调节因子

组蛋白的翻译后修饰对于记忆形成至关重要。最近，我们发现组蛋白 H2B 在赖氨酸 120 (H2Bub) 处的单泛素化对于海马记忆形成至关重要。然而，H2Bub 控制的转录组仍然未知。在这里，我们发现雄性大鼠的恐惧条件反射增加或减少了海马中 86 个基因的表达，但令人惊讶的是，siRNA 介导的 H2Bub 连接酶Rnf20的敲低消除了除其中一个基因之外的所有基因的变化。这些发现表明组蛋白 H2B 的单泛素化是记忆形成过程中转录组的关键调节因子。