Three novel, Gram-stain-positive, obligate aerobic, catalase- and oxidase-positive bacterial strains, designated B2O-1^T, T2O-4^T, and 0.2-SM1T-5^T, were isolated from jeotgal, a traditional Korean fermented seafood. Strains B2O-1^T, T2O-4^T, and 0.2-SM1T-5^T exhibited distinct colony colors, characterized by pink, yellow, and red opaque circular colonies, respectively. Phylogenetic analysis revealed that three strains formed a paraphyletic clade within the genus Sporosarcina and shared < 99.0% similarity with Sporosarcina aquimarina KCTC 3840^T and Sporosarcina saromensis KCTC 13119^T in their 16S rRNA gene sequences. The three strains exhibiting Orthologous Average Nucleotide Identity values < 79.3% and digital DNA-DNA hybridization values < 23.1% within the genus Sporosarcina affirmed their distinctiveness. Strains B2O-1^T, T2O-4^T, and 0.2-SM1T-5^T contained MK-7 as a sole respiratory menaquinone and A4α type peptidoglycan based on lysine with alanine, glutamic acid, and aspartic acid. The common polar lipids include diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Strain T2O-4^T contained one unidentified phospholipid, whereas strain 0.2-SM1T-5^T contained two unidentified phospholipids. Cellular fatty acid profiles, with C_15:0 anteiso as the major fatty acid, supported the affiliation of the three strains to the genus Sporosarcina. Based on the polyphasic characteristics, strains B2O-1^T (= KCTC 43506^T = JCM 36032^T), T2O-4^T (= KCTC 43489^T = JCM 36031^T), and 0.2-SM1T-5^T (= KCTC 43519^T = JCM 36034^T) represent three novel species within the genus Sporosarcina, named Sporosarcina jeotgali sp. nov., Sporosarcina oncorhynchi sp. nov., and Sporosarcina trichiuri sp. nov., respectively.

从韩国传统发酵海鲜 jeotgal 中分离出三种新型革兰氏染色阳性、专性需氧、过氧化氢酶和氧化酶阳性细菌菌株，命名为 B2O-1 ^T、 T2O-4 ^T和 0.2-SM1T-5 ^T。 。菌株 B2O-1 ^T、 T2O-4 ^T和 0.2-SM1T-5 ^T表现出不同的菌落颜色，分别以粉红色、黄色和红色不透明圆形菌落为特征。系统发育分析显示，三个菌株在孢子八叠菌属内形成了一个并系进化枝，其 16S rRNA 基因序列与Sporosarcina aquimarina KCTC 3840 ^T和Sporosarcina saromensis KCTC 13119 ^T的相似性低于 99.0% 。孢子八叠球菌属内的三个菌株表现出直系同源平均核苷酸同一性值 < 79.3% 和数字 DNA-DNA 杂交值 < 23.1%，证实了它们的独特性。菌株 B2O-1 ^T、T2O-4 ^T和 0.2-SM1T-5 ^T含有作为唯一呼吸性甲基萘醌的 MK-7 和基于赖氨酸、丙氨酸、谷氨酸和天冬氨酸的A4 α型肽聚糖。常见的极性脂质包括二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。菌株T2O-4 ^T含有一种未鉴定的磷脂，而菌株0.2-SM1T-5 ^T含有两种未鉴定的磷脂。细胞脂肪酸谱（以 C _15:0 anteiso 作为主要脂肪酸）支持这三个菌株属于孢子八叠球菌属。根据多相特征，菌株 B2O-1 ^T (= KCTC 43506 ^T  = JCM 36032 ^T )、T2O-4 ^T (= KCTC 43489 ^T  = JCM 36031 ^T ) 和 0.2-SM1T-5 ^T (= KCTC 43519 ^T  = JCM 36034 ^{T )代表孢子}八菌属内的三个新物种，命名为Sporosarcina jeotgali sp。十一月，Sporosarcina oncorhynchi sp。 11 月，和Sporosarcina trichiuri sp。十一月，分别。