Proteases, such as trypsin, are essential for extracting collagen in various industrial applications. The potential applications of rare earth nanomaterials, specifically yttrium nanoparticles, have attracted significant interest across various fields due to their distinctive characteristics, including high dielectric constant and thermal stability. Biomineralization has emerged as a promising approach to synthesize protein-inorganic nanomaterials with hierarchical structures and desired functions. In the present investigation, a novel protease-templated biomineralization strategy was developed for synthesizing protease-(NH4)2Y3F11•H2O hybrid nanomaterials using a one-pot method under very mild conditions. For modifying the morphologies of (NH4)2Y3F11•H2O throughout biomineralization, protease has been demonstrated to be a highly promising biotemplate. Protease was utilized as a template for morphological control in the biomineralization procedure, which resulted in a gradual transformation of the initially formed (NH4)2Y3F11•H2O octahedral structures into uniform nanospheres. The applicability of this approach was supported by successfully utilizing various proteases to synthesize protease-(NH4)2Y3F11•H2O hybrid nanospheres. In addition to a strong and desirable luminescent signal, these hybrid nanospheres demonstrated extensive recycling because of their high enzymatic activity, stability and durability. The protease-mediated biomineralization approach offers an easy and robust approach to develop innovative protease-inorganic composites. Its moderate reaction conditions and simple operation render it a viable tool for developing stable and reusable enzyme reactors in various industrial applications.

中文翻译：

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发光蛋白酶-(NH4)2Y3F11·H2O杂化纳米球的生物矿化合成及其作为稳定、可重复使用的酶反应器的应用

蛋白酶（例如胰蛋白酶）对于在各种工业应用中提取胶原蛋白至关重要。稀土纳米材料（特别是钇纳米颗粒）的潜在应用由于其独特的特性（包括高介电常数和热稳定性）而引起了各个领域的极大兴趣。生物矿化已成为合成具有分层结构和所需功能的蛋白质无机纳米材料的一种有前途的方法。在本研究中，开发了一种新型蛋白酶模板生物矿化策略，用于在非常温和的条件下使用一锅法合成蛋白酶-(NH4)2Y3F11·H2O杂化纳米材料。为了在整个生物矿化过程中改变(NH4)2Y3F11·H2O的形态，蛋白酶已被证明是一种非常有前途的生物模板。蛋白酶被用作生物矿化过程中形态控制的模板，导致最初形成的(NH4)2Y3F11·H2O八面体结构逐渐转变为均匀的纳米球。成功地利用各种蛋白酶合成了蛋白酶-(NH4)2Y3F11•H2O杂化纳米球，证明了该方法的适用性。除了强烈且理想的发光信号之外，这些混合纳米球还因其高酶活性、稳定性和耐用性而表现出广泛的回收利用。蛋白酶介导的生物矿化方法提供了一种简单而可靠的方法来开发创新的蛋白酶-无机复合材料。其温和的反应条件和简单的操作使其成为在各种工业应用中开发稳定且可重复使用的酶反应器的可行工具。