In recent years, low-density polyethylene (LDPE) has emerged as an essential component of the routine tasks that people engage in on a daily basis. However, over use of it resulted in environmental buildup that contaminated aquatic habitats and human health. Biodegradation is the most effective way for controlling pollution caused by synthetic plastic waste in a sustainable manner. In the present study, the LDPE degrading bacterial strain was screened from gut of Earthworms collected from plastic waste dumped area Mettur dam, Salem district, Tamil Nadu, India. The LDPE degrading bacterial strain was screened and identified genotypically. The LDPE degrading Bacillus gaemokensis strain SSR01 was submitted in NCBI. The B. gaemokensis strain SSR01 bacterial isolate degraded LDPE film after 14 days of incubation and demonstrated maximum weight loss of up to 4.98%. The study of deteriorated film using attenuated total reflection-Fourier transform infrared revealed the presence of a degraded product. The degradation of LDPE film by B. gaemokensis strain SSR01 was characterized by field-emission scanning electron microscopy analysis for surface alterations. The energy dispersive X-ray spectroscopy test confirmed that the broken-down LDPE film had basic carbon reduction. The present study of LDPE flim biodegradation by B. gaemokensis strain SSR01 has acted as a suitable candidate and will help in decreasing plastic waste.

Graphical abstract

中文翻译：

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蚯蚓肠道中分离的 Bacillus gaemokensis 菌株 SSR01 对低密度聚乙烯膜的生物降解

近年来，低密度聚乙烯（LDPE）已成为人们日常工作的重要组成部分。然而，过度使用会导致环境恶化，污染水生栖息地和人类健康。生物降解是以可持续的方式控制合成塑料废物造成的污染的最有效方法。在本研究中，从印度泰米尔纳德邦塞勒姆区 Mettur 水坝塑料废物倾倒区收集的蚯蚓肠道中筛选出 LDPE 降解菌株。对LDPE降解菌株进行了筛选和基因型鉴定。 LDPE降解芽孢杆菌菌株SSR01已提交至NCBI。孵育 14 天后， B . gaemokensis菌株 SSR01 细菌分离株可降解 LDPE 薄膜，并表现出最大重量损失高达 4.98%。使用衰减全反射傅立叶变换红外对劣化薄膜进行的研究揭示了劣化产物的存在。通过场发射扫描电子显微镜分析表面变化来表征B. gaemokensis菌株 SSR01对 LDPE 薄膜的降解。能量色散X射线光谱测试证实，断裂的LDPE薄膜发生了碱性碳还原。目前对B. gaemokensis菌株 SSR01进行 LDPE 薄膜生物降解的研究已成为合适的候选者，将有助于减少塑料废物。

图形概要