Cyantraniliprole (CY), an anthranilic diamide insecticide widely used in grape farming for controlling various sucking pests, poses ecological concerns, particularly when applied as soil drenching due to the formation of more toxic and persistent metabolites. This study established the dissipation and degradation mechanisms of CY in grape rhizosphere soil using high-resolution Orbitrap-LC/MS analysis. The persistence of CY residues beyond 60 days was observed, with dissipation following biphasic first + first-order kinetics and a half-life of 15 to 21 days. The degradation mechanism of CY in the soil was elucidated, with identified metabolites such as IN-J9Z38, IN-JCZ38, IN-N7B69, and IN-QKV54. Notably, CY was found to predominantly convert to the highly persistent metabolite IN-J9Z38, raising environmental concerns. The impact of CY residues on soil enzyme activity was investigated, revealing a negative effect on dehydrogenase, alkaline phosphatase, and acid phosphatase activity, indicating significant implications for phosphorous mineralization and soil health. Furthermore, bacterial isolates were obtained from CY-enriched soil, with five isolates (CY3, CY4, CY9, CY11, and CY20) demonstrating substantial degradation potential, ranging from 66 to 92% of CY residues. These results indicate that the identified bacteria hold potential for commercial use in addressing pesticide residue contamination in soil through bioremediation techniques.

氰虫酰胺 (CY) 是一种邻氨基苯甲酸二酰胺杀虫剂，广泛用于葡萄种植中，用于控制各种刺吸式害虫，但它会引起生态问题，特别是在土壤浇灌时，会形成毒性更强、更持久的代谢物。本研究利用高分辨率 Orbitrap-LC/MS 分析建立了 CY 在葡萄根际土壤中的消散和降解机制。观察到 CY 残基的持续存在超过 60 天，并遵循双相一级 + 一级动力学消散，半衰期为 15 至 21 天。阐明了 CY 在土壤中的降解机制，并鉴定出 IN-J9Z38、IN-JCZ38、IN-N7B69 和 IN-QKV54 等代谢物。值得注意的是，CY 主要转化为高度持久的代谢物 IN-J9Z38，引起了环境问题。研究了 CY 残留物对土壤酶活性的影响，揭示了对脱氢酶、碱性磷酸酶和酸性磷酸酶活性的负面影响，表明对磷矿化和土壤健康具有重大影响。此外，从富含 CY 的土壤中获得细菌分离株，其中 5 个分离株（CY3、CY4、CY9、CY11 和 CY20）表现出巨大的降解潜力，其 CY 残留量的范围为 66% 至 92%。这些结果表明，所鉴定的细菌具有通过生物修复技术解决土壤中农药残留污染的商业用途的潜力。