BackgroundTumor initiation and metastasis influence tumor immune exclusion and immunosuppression. Long non-coding RNA (lncRNA) LINC01614 is associated with the prognosis and metastasis of several cancers. However, the relationship between LINC01614 and cancer immune infiltration and the biofunction of LINC01614 in head and neck squamous cell carcinoma (HNSC) remain unclear.MethodsThe Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) datasets were used to analyze the expression difference and diagnostic value of LINC01614 in normal and tumor tissues. The correlation of pan-cancer prognosis and tumor stage of LINC01614 was analyzed based on the TCGA database. The pan-cancer association of LINC01614 expression with the tumor microenvironment (TME) including immune infiltration, expression of immune-related genes, and genomic instability parameters, was analyzed using the Spearman correlation method. The correlation between LINC01614 and tumor stemness evaluation indicators, RNA methylation-related genes, and drug resistance was also analyzed. The functional analysis of LINC01614 was performed using the clusterProfiler R package. The protein–protein interaction (PPI) network and ceRNA network of LINC01614 co-expressed genes and miRNA were constructed and visualized by STRING and Cytoscape, respectively. Finally, the cell location and influence of LINC01614 on cell proliferation and metastasis of HNSC cell lines were evaluated using FISH, CCK-8, wound-healing assay, and transwell assay.ResultsLINC01614 was found to be overexpressed in 23 cancers and showed a highly sensitive prediction value in nine cancers (AUC >0.85). LINC01614 dysregulation was associated with tumor stage in 12 cancers and significantly influenced the survival outcomes of 26 cancer types, with only Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (DLBC), uterine corpus endometrial carcinoma (UCEC), and bladder urothelial carcinoma (BLCA) showing a benign influence. LINC01614 was also associated with immune cell infiltration, tumor heterogeneity, cancer stemness, RNA methylation modification, and drug resistance. The potential biological function of LINC01614 was verified in HNSC, and it was found to play important roles in proliferation, immune infiltration, immunotherapy response, and metastasis of HNSC.ConclusionLINC01614 may serve as a cancer diagnosis and prognosis biomarker and an immunotherapy target for specific cancers.

中文翻译：

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LINC01614 是一种有前景的 HNSC 诊断和预后标记物，与肿瘤微环境和致癌功能相关

研究背景肿瘤的发生和转移影响肿瘤的免疫排斥和免疫抑制。长非编码 RNA (lncRNA) LINC01614 与多种癌症的预后和转移相关。然而，LINC01614与癌症免疫浸润的关系以及LINC01614在头颈鳞状细胞癌（HNSC）中的生物功能仍不清楚。方法利用基因型-组织表达（GTEx）和癌症基因组图谱（TCGA）数据集分析LINC01614在正常组织和肿瘤组织中的表达差异及诊断价值基于TCGA数据库分析LINC01614的全癌预后与肿瘤分期的相关性。使用 Spearman 相关方法分析 LINC01614 表达与肿瘤微环境 (TME) 的泛癌关联，包括免疫浸润、免疫相关基因的表达和基因组不稳定参数。还分析了LINC01614与肿瘤干性评价指标、RNA甲基化相关基因、耐药性的相关性。 LINC01614 的功能分析是使用 clusterProfiler R 包进行的。分别通过 STRING 和 Cytoscape 构建并可视化 LINC01614 共表达基因和 miRNA 的蛋白质-蛋白质相互作用 (PPI) 网络和 ceRNA 网络。最后，使用FISH、CCK-8、伤口愈合实验和transwell实验评估LINC01614的细胞定位以及对HNSC细胞系的细胞增殖和转移的影响。结果发现LINC01614在23种癌症中过表达，并表现出高度的敏感性。九种癌症的预测值(AUC＞0.85)。 LINC01614 失调与 12 种癌症的肿瘤分期相关，并显着影响 26 种癌症类型的生存结果，其中只有淋巴肿瘤弥漫性大 B 细胞淋巴瘤 (DLBC)、子宫体子宫内膜癌 (UCEC) 和膀胱尿路上皮癌 (BLCA)呈现出良性影响。 LINC01614 还与免疫细胞浸润、肿瘤异质性、癌症干性、RNA 甲基化修饰和耐药性相关。 LINC01614的潜在生物学功能在HNSC中得到验证，发现其在HNSC的增殖、免疫浸润、免疫治疗反应和转移中发挥重要作用。结论LINC01614可作为癌症诊断和预后的生物标志物以及特定癌症的免疫治疗靶点。