Lentil is one of the most important legumes cultivated in various provinces of Iran. However, there is limited information about the symbiotic rhizobia of lentils in this country. In this study, molecular identification of lentil-nodulating rhizobia was performed based on 16S-23S rRNA intergenic spacer (IGS) and recA, atpD, glnII, and nodC gene sequencing. Using PCR-RFLP analysis of 16S-23S rRNA IGS, a total of 116 rhizobia isolates were classified into 20 groups, leaving seven strains unclustered. Phylogenetic analysis of representative isolates revealed that the rhizobia strains belonged to Rhizobium leguminosarum and Rhizobium laguerreae, and the distribution of the species is partially related to geographical location. R. leguminosarum was the dominant species in North Khorasan and Zanjan, while R. laguerreae prevailed in Ardabil and East Azarbaijan. The distribution of the species was also influenced by agroecological climates; R. leguminosarum thrived in cold semi-arid climates, whereas R. laguerreae adapted to humid continental climates. Both species exhibited equal dominance in the Mediterranean climate, characterized by warm, dry summers and mild, wet winters, in Lorestan and Kohgiluyeh-Boyer Ahmad provinces.

中文翻译：

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伊朗扁豆结瘤根瘤菌的地理和气候分布

小扁豆是伊朗各省种植的最重要的豆类之一。然而，有关该国扁豆共生根瘤菌的信息有限。在本研究中，基于 16S-23S rRNA 基因间间隔区 (IGS) 和 recA、atpD、glnII 和 nodC 基因测序对扁豆结瘤根瘤菌进行了分子鉴定。通过对 16S-23S rRNA IGS 进行 PCR-RFLP 分析，总共 116 个根瘤菌分离株被分为 20 个组，其中 7 个菌株未聚类。对代表性菌株的系统发育分析表明，根瘤菌属于豆根瘤菌和拉盖尔根瘤菌，其物种分布与地理位置部分相关。豆豆根瘤菌是北呼罗珊和赞詹的优势种，而拉盖尔根瘤菌在阿尔达比勒和东阿塞拜疆占主导地位。该物种的分布也受到农业生态气候的影响；豆角杆菌在寒冷的半干旱气候中繁衍生息，而拉盖雷角杆菌则适应潮湿的大陆性气候。这两个物种在洛雷斯坦省和科吉卢耶-博耶艾哈迈德省的地中海气候中表现出同等的优势，其特点是夏季温暖干燥，冬季温和潮湿。