Objective

In this study, we investigated the impact of perioperative administration of Bifidobacterium triplex viable capsules on the serum levels of circulating miR-21-5p, miR-135-5p, and miR-155-5p in patients with colorectal cancer (CRC). The purpose of this study is to provide a foundation for future research on the use of Bifidobacterium triplex viable capsules to enhance postoperative recovery in patients with CRC.

Methods

A total of 60 patients with primary CRC admitted to the Department of General Surgery at Shanxi Bethune Hospital between June 2020 and December 2020 were selected and randomly divided into two groups: 20 cases in the control group and 40 cases in the experimental group. The experimental group was administered oral Bifidobacterium triplex viable capsules during the perioperative period, while the control group was administered oral placebo. Before and after the perioperative period, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p were compared in the serum of both groups of patients. Furthermore, we established the prognostic value of these three miRNAs in CRC patients.

Results

After surgery, the expression levels of miR-21-5p, miR-135-5p, and miR-155-5p decreased in both groups of patients (P < 0.05). Significantly greater differences were observed between miR-21-5p and miR-135-5p (P < 0.001). Expression levels of serum miR-21-5p (P = 0.020) and miR-135-5p (P = 0.023) decreased significantly more in the experimental group than in the control group. The levels of the above three miRNAs after surgery did not correlate with 3-year OS (HR = 4.21; 95% CI 0.37–47.48; log-rank P = 0.20) or 3-year DFS (HR = 1.57; 95% CI 0.32–7.66; log-rank P = 0.55) in two groups.

Conclusion

Radical surgery reduces the levels of serum miR-21-5p, miR-135-5p, and miR-155-5p expression in patients with CRC. The use of Bifidobacterium triplex viable capsules assists in achieving quicker perioperative recovery from radical surgery in CRC patients, and this underlying mechanism may be associated with the regulation of serum miR-21-5p, miR-135-5p, and miR-155-5p expression levels.

中文翻译：

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双歧杆菌三联活菌胶囊对结直肠癌围术期血清miR-21-5p、miR-135-5p、miR-155-5p差异表达分析

客观的

在这项研究中，我们研究了围手术期服用双歧杆菌三联活菌胶囊对结直肠癌 (CRC) 患者循环 miR-21-5p、miR-135-5p 和 miR-155-5p 血清水平的影响。本研究的目的是为未来使用双歧杆菌三联活菌胶囊促进结直肠癌患者术后恢复的研究奠定基础。

方法

选择2020年6月至2020年12月山西白求恩医院普外科收治的60例原发性CRC患者，随机分为两组：对照组20例，实验组40例。实验组围手术期口服双歧杆菌三联活菌胶囊，对照组口服安慰剂。围手术期前后比较两组患者血清中miR-21-5p、miR-135-5p、miR-155-5p的表达量。此外，我们还确定了这三种 miRNA 在 CRC 患者中的预后价值。

结果

术后，两组患者 miR-21-5p、miR-135-5p、miR-155-5p 表达量均下降（P  < 0.05）。 miR-21-5p 和 miR-135-5p 之间观察到显着更大的差异（P  < 0.001）。实验组血清中miR-21-5p（ P  =0.020）和miR-135-5p（P =0.023）表达水平较 对照组显着下降。手术后上述三种 miRNA 的水平与 3 年 OS（HR = 4.21；95% CI 0.37–47.48；对数秩P  = 0.20）或 3 年 DFS（HR = 1.57；95% CI 0.32）不相关。 –7.66；对数秩P  = 0.55) 两组。

结论

根治性手术降低了 CRC 患者血清 miR-21-5p、miR-135-5p 和 miR-155-5p 的表达水平。双歧杆菌三联活菌胶囊的使用有助于结直肠癌根治性手术患者更快地实现围术期恢复，其潜在机制可能与血清 miR-21-5p、miR-135-5p 和 miR-155-5p 的调节有关表达水平。