In the era of genomic-assisted breeding for crop improvement, developing new molecular markers and validating them for use in breeding programs are the prelude. Dolichos bean is one of the most important vegetable legume crops owing to its nutrient-rich green pods used as vegetables. Limitations in genomic resources, including molecular markers, restrict the accelerated improvement of the crop. In the present investigation, a set of 430 new simple sequence repeat markers was developed from sequence information of a reference variety. These markers included di- and tri-nucleotide repeats. The markers were assayed on an association panel, which was evaluated for green pod yield over 5 years. A multi-locus model, FarmCPU, was used to assess the marker-trait association analysis. A total of 106 marker-trait associations were identified using an efficient mixed-model approach. Tri-nucleotide repeats were more informative and predominantly associated with trait. Among these markers, 17 were associated with a high level of significance. Markers LP-D-68 and LP-D-14 were identified with a high level of significance in 5-year pooled data and explained 12.70% and 12% of the phenotypic variance, respectively. These markers associated with a high level of confidence have significant scope for use in marker-assisted selection programmes. Other associated markers may be utilized for improving parents through marker-assisted recurrent selection or genomic selection programs.

在作物改良的基因组辅助育种时代，开发新的分子标记并验证它们在育种计划中的使用是前奏。扁豆因其营养丰富的绿色豆荚用作蔬菜而成为最重要的蔬菜豆类作物之一。包括分子标记在内的基因组资源的限制限制了作物的加速改良。在本次调查中，根据参考品种的序列信息开发了一组 430 个新的简单序列重复标记。这些标记包括二核苷酸和三核苷酸重复。在关联面板上对标记物进行分析，并评估 5 年内的绿荚产量。使用多位点模型 FarmCPU 来评估标记-性状关联分析。使用有效的混合模型方法总共鉴定了 106 个标记-性状关联。三核苷酸重复信息更丰富，并且主要与性状相关。在这些标记中，有 17 个具有高度显着性。标记物 LP-D-68 和 LP-D-14 在 5 年汇总数据中具有高度显着性，分别解释了 12.70% 和 12% 的表型方差。这些与高置信度相关的标记在标记辅助选择计划中具有很大的使用范围。其他相关标记可用于通过标记辅助轮回选择或基因组选择程序来改善亲本。