Quantitative phase imaging plays a crucial role in biological imaging, with many phase retrieval technologies being particularly important. However, cells and tissues must be placed on certain carriers during imaging, such as glass slides, cell culture dishes, and cell culture boxes. Therefore, current unidirectional phase retrieval technologies are not suitable for biplane samples, causing the phase of the object to always be coupled with the phase of noise. As a solution, this study proposes bidirectional phase retrieval to independently analyze the phase of both planes in the sample. Considering that most biological samples can be treated as two planes: one representing the object and the other serving as noise. By decoupling the phase of the two planes, this approach protects biplane biological samples from interference caused by the noise plane, leading to more accurate phase retrieval. This innovation holds significant value for the advancement of biological imaging.

中文翻译：

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双向相位检索：保护细胞和组织的成像免受载体上噪声的干扰

定量相位成像在生物成像中起着至关重要的作用，其中许多相位检索技术尤其重要。然而，在成像过程中，细胞和组织必须放置在某些载体上，例如载玻片、细胞培养皿和细胞培养盒。因此，目前的单向相位检索技术并不适合双平面样本，导致物体的相位总是与噪声的相位耦合。作为解决方案，本研究提出了双向相位检索来独立分析样本中两个平面的相位。考虑到大多数生物样本可以被视为两个平面：一个代表对象，另一个作为噪声。通过解耦两个平面的相位，该方法可以保护双平面生物样本免受噪声平面造成的干扰，从而实现更准确的相位检索。这项创新对于生物成像的进步具有重要价值。