Background: Platelets are a commonly used blood component to prevent or treat bleeding in patients with thrombocytopenia or platelet dysfunction. They are stored at room temperature (22– 24°C) for five days unless specific measures are taken to extend the shelf life to seven days or more. After five days, this study evaluated platelet units’ biochemical changes and bacterial growth.
Study Design and Methods: Platelet concentrate was collected from 30 random donors: 8 females and 22 males. The collected samples were then placed on an agitator at room temperature and tested for their pH, protein content, and glucose levels using Roche Combur 100 Test^® Strips. The Haemonetics eBDS™ System was used for bacterial detection. The measurements were taken on day five as the control and then repeated on days 7, 9, and 11 to observe any changes. On days 5 and 7, all parameters remained unchanged. However, glucose levels significantly changed (p=< 0.0001) on days 9 and 11. Regarding pH, a significant change was observed on day 9 (p=0.033) and day 11 (p=0.0002).
Results: There were no significant changes in all parameters on days 5 and 7. However, glucose was substantially changed (p=< 0.0001) on days 9 and 11. For pH, there was a significant change in pH on day 9 (p=0.033) and day 11 (p=0.0002).
Discussions: Our study found that platelet concentrate extension is possible for up to seven days. However, further studies are needed to evaluate platelet function during expiry time and to assess the stability of platelet morphology and function.



中文翻译：

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过期浓缩血小板的化学和微生物变化

背景：血小板是一种常用的血液成分，用于预防或治疗血小板减少或血小板功能障碍患者的出血。除非采取特殊措施将保质期延长至 7 天或更长，否则它们会在室温 (22–24°C) 下保存五天。五天后，这项研究评估了血小板单位的生化变化和细菌生长。
研究设计和方法：从 30 名随机捐赠者中收集浓缩血小板：8 名女性和 22 名男性。然后将收集的样品置于室温下的搅拌器上，并使用 Roche Combur 100 Test ^® Strips 测试其 pH 值、蛋白质含量和葡萄糖水平。 Haemonetics eBDS™ 系统用于细菌检测。第五天进行测量作为对照，然后在第 7、9 和 11 天重复测量以观察任何变化。第 5 天和第 7 天，所有参数均保持不变。然而，葡萄糖水平在第 9 天和第 11 天发生显着变化 (p=< 0.0001)。关于 pH 值，在第 9 天 (p=0.033) 和第 11 天 (p=0.0002) 观察到显着变化。
结果：第 5 天和第 7 天的所有参数均没有显着变化。然而，第 9 天和第 11 天的葡萄糖发生了显着变化 (p=< 0.0001)。对于 pH 值，第 9 天的 pH 值发生了显着变化 (p= 0.033）和第 11 天（p=0.0002）。
讨论：我们的研究发现浓缩血小板的延长时间最多可达 7 天。然而，需要进一步的研究来评估有效期内的血小板功能以及评估血小板形态和功能的稳定性。