Caves are unique habitats, ranging from well-illuminated to devoid of light environments that host various forms of life. Among the microbial groups that can inhabit these environments across different lithologies are lactic acid bacteria (LAB), a widely distributed group of bacteria that are poorly understood in cave environments. In this study, samples from limestone and sandstone caves were analyzed to determine the presence of LAB using two group-specific primer pairs. Only one of the primer pairs amplified, and the DGGE analysis showed a profile with a few bands for each sample, except for the entrance and the first inner sample from the metasandstone cave, and one inner sample from the limestone cave, which had a higher number of operational taxonomic units (OTUs). Sequencing of selected OTUs revealed the presence of Streptococcus and Lactococcus. Energy-dispersive X-ray spectroscopy showed that the inner sample from the limestone cave with a higher number of OTUs also had a higher percentage of magnesium. Despite efforts to cultivate LAB using common culture media such as MRS and GYP, no growth was observed, suggesting that these media may not be suitable for isolating LAB from cave sediments and rocks. The presence of this group in all nine samples analyzed from caves highlights the significance of these microorganisms and suggests the need for further research to cultivate and understand their role in the cave microbial community and their biotechnological potential.

洞穴是独特的栖息地，从光照充足到缺乏光照的环境都适合各种生命形式。乳酸菌（LAB）是可以在不同岩性环境中栖息的微生物群之一，这是一种分布广泛的细菌群，但人们对洞穴环境中的了解知之甚少。在这项研究中，分析了来自石灰岩和砂岩洞穴的样品，使用两对特定的引物对来确定 LAB 的存在。仅扩增了一对引物，DGGE 分析显示每个样品的轮廓都有几个条带，除了变砂岩洞穴的入口和第一个内部样品，以及来自石灰岩洞穴的一个内部样品，其具有较高的条带。操作分类单元（OTU）的数量。对选定 OTU 的测序揭示了链球菌和乳球菌的存在。能量色散 X 射线光谱显示，来自 OTU 数量较多的石灰岩洞穴内部样品的镁含量也较高。尽管努力使用 MRS 和 GYP 等常见培养基培养 LAB，但没有观察到生长，这表明这些培养基可能不适合从洞穴沉积物和岩石中分离 LAB。从洞穴中分析的所有九个样本中都存在该群体，这凸显了这些微生物的重要性，并表明需要进一步研究来培养和了解它们在洞穴微生物群落中的作用及其生物技术潜力。