Current mesh materials used for the clinical treatment of abdominal defects struggle to balance mechanical properties and bioactivity to support tissue remodeling. Therefore, a bioactive microgel-coated electrospinning membrane was designed with the superiority of cell-instructive topology in guiding cell behavior and function for abdominal wall defect reconstruction. The electrostatic spinning technique was employed to prepare a bioabsorbable PLCL fiber membrane with an effective mechanical support. Additionally, decellularized matrix (dECM)-derived bioactive microgels were further coated on the fiber membrane through co-precipitation with dopamine, which was expected to endow cell-instructive hydrophilic interfaces and topological morphologies for cell adhesion. Moreover, the introduction of the dECM into the microgel promoted the myogenic proliferation and differentiation of C2C12 cells. Subsequently, in vivo experiments using a rat abdominal wall defect model demonstrated that the bioactive microgel coating significantly contributed to the reconstruction of intact abdominal wall structures, highlighting its potential for clinical application in promoting the repair of soft tissue defects associated with abdominal wall damage. This study presented an effective mesh material for facilitating the reconstruction of abdominal wall defects and contributed novel design concepts for the surface modification of scaffolds with cell-instructive interfaces and topology.

中文翻译：

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具有细胞指导界面和拓扑结构的生物活性微凝胶涂层电纺膜，用于修复腹壁缺损

目前用于临床治疗腹部缺陷的网状材料难以平衡机械性能和生物活性以支持组织重塑。因此，设计了一种生物活性微凝胶涂层静电纺丝膜，其具有细胞指导拓扑的优越性，可指导细胞行为和功能用于腹壁缺损重建。采用静电纺丝技术制备了具有有效机械支撑的生物可吸收PLCL纤维膜。此外，通过与多巴胺共沉淀，将脱细胞基质（dECM）衍生的生物活性微凝胶进一步涂覆在纤维膜上，这有望赋予细胞指导性的亲水界面和细胞粘附的拓扑形态。此外，将dECM引入微凝胶中促进了C2C12细胞的生肌增殖和分化。随后，使用大鼠腹壁缺损模型进行的体内实验表明，生物活性微凝胶涂层对完整腹壁结构的重建有显着贡献，突显了其在促进与腹壁损伤相关的软组织缺损修复方面的临床应用潜力。这项研究提出了一种有效的网状材料，可促进腹壁缺损的重建，并为具有细胞指导界面和拓扑结构的支架表面修饰提供了新颖的设计理念。