We describe S_NAr reactions of some commercial amino-tetrazines and halo-dyes, which give efficiently quenched BODIPY/NBD–tetrazines (Φ_Fl < 0.01) in high yields and, importantly, with high purities affordable via simple silica gel chromatography only. The dyes exhibit large Stokes shifts, moderate environmental sensitivity, and emission enhancements (up to 193-fold) upon Tz ligation with BCN─a strained dienophile. They successfully serve as labels for HSA protein premodified with BCN, resulting in bright blue–green emission upon ligation.

中文翻译：

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通过 SNAr 反应可轻松获得且明亮的荧光 BODIPY/NBD-四嗪

我们描述了一些商业氨基四嗪和卤代染料的 SNA Ar 反应，这些反应以高产率有效淬灭 BODIPY/NBD-四嗪 ( _Φ Fl _< 0.01)，重要的是，仅通过简单的硅胶色谱即可获得高纯度。这些染料在与 BCN（一种应变亲双烯体）Tz 连接后表现出较大的斯托克斯位移、中等环境敏感性和发射增强（高达 193 倍）。它们成功地作为用 BCN 预修饰的 HSA 蛋白的标记，在连接时产生明亮的蓝绿色发射。