(DHPV) is a single-stranded DNA virus that primarily affects the early life stages of penaeid shrimp, potentially leading to significant losses in hatchery operations. In an interesting finding, we observed that a small portion Black tiger shrimp () originating from Madagascar harbored DHPV even in the absence of any discernible clinical signs. Detailed histopathological analysis of post-larvae individuals revealed an absence of distinctive histological lesions typically associated with DHPV infection. However, sensitive molecular techniques, including PCR and real-time PCR and reverse transcriptase quantitative PCR (RT-qPCR) confirmed the presence of viral DNA and DHPV encoded transcripts in these specimens. The complete genome sequence of this newfound viral isolate, spanning 6254 nucleotides, showed a 96.9% nucleotide sequence similarity to a previously documented DHPV strain also originating from Madagascar. Moreover, it exhibited a genetic resemblance of 80.7% to 88.2% when compared to DHPV strains from other geographical regions. These findings underscore the unique genomic and histological characteristics of this novel DHPV isolate. This knowledge proves to be of paramount significance for the screening of broodstock and post-larvae in captive breeding programs, as it aids in identifying the presence of DHPV. Furthermore, it paves the way for future investigations into unraveling the precise role of viral-encoded proteins in shaping the clinical and histological manifestations of DHPV infections.

中文翻译：

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马达加斯加黑虎虾（斑节对虾）中不引起组织学损伤的十足目肝炎细小病毒新毒株的鉴定和表征

(DHPV) 是一种单链 DNA 病毒，主要影响对虾生命的早期阶段，可能导致孵化场运营遭受重大损失。在一项有趣的发现中，我们观察到一小部分源自马达加斯加的黑虎虾携带 DHPV，即使没有任何明显的临床症状。对后期幼虫个体的详细组织病理学分析显示，不存在通常与 DHPV 感染相关的独特组织学病变。然而，敏感的分子技术，包括 PCR、实时 PCR 和逆转录酶定量 PCR (RT-qPCR)，证实这些样本中存在病毒 DNA 和 DHPV 编码的转录本。这种新发现的病毒分离株的完整基因组序列涵盖 6254 个核苷酸，与先前记录的同样源自马达加斯加的 DHPV 毒株有 96.9% 的核苷酸序列相似性。此外，与其他地理区域的 DHPV 毒株相比，它表现出 80.7% 至 88.2% 的遗传相似性。这些发现强调了这种新型 DHPV 分离株独特的基因组和组织学特征。事实证明，这些知识对于圈养繁殖计划中亲体和后期幼体的筛查至关重要，因为它有助于识别 DHPV 的存在。此外，它为未来研究揭示病毒编码蛋白在 DHPV 感染的临床和组织学表现中的精确作用铺平了道路。