With an increase in the generation of sewage sludge, there comes a demand for disposal methods for it, and agricultural use is a sustainable alternative. However, there are gaps regarding emerging pollutants, especially in the pharmaceutical class. Nimesulide - an anti-inflammatory pharmaceutical recently associated with liver damage and banned in some European countries - is among the most consumed pharmaceuticals in Brazil hence can be found in wastewater and, therefore, in sewage sludge used for agriculture at ng L concentrations. The objective of this research was to evaluate the toxicity of the anti-inflammatory pharmaceutical nimesulide through toxicity tests using the organism in both natural and artificial soils, adopting concentrations about studies carried out with other anti-inflammatory drugs on seeds in the soil. In this work, the toxicity of nimesulide was evaluated in natural tropical soil sampled in São Paulo – Brazil, and in tropical artificial soil. The invertebrate was exposed to nimesulide nominal concentrations of 200; 340; 578; 982 and 1670 mg nimesulide kg of dry soil and the reproduction test was performed according to ISO 16.387 (2014) standard, with 5 replicates per concentration. The results obtained through reproduction testing with in each concentration presented were EC50 values of 699 and 759 mg nimesulide Kg dry soil, respectively for both natural and artificial soil. The Tukey test showed no significant difference between the soils, however. Finally, this study suggests that the anti-inflammatory drug nimesulide displays a toxicological effect on the reproduction of soil fauna organisms in high concentrations. Therefore, stresses the importance of identifying and monitoring unknown concentrations of this emerging pollutant in the environment.

中文翻译：

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使用土壤动物 Enchytraeus crypticus 评估药物尼美舒利的土壤毒性

随着污水污泥产生量的增加，对其处理方法提出了需求，农业利用是一种可持续的替代方案。然而，在新兴污染物方面还存在差距，特别是在制药类污染物方面。尼美舒利是一种抗炎药物，最近与肝损伤有关，在一些欧洲国家被禁止。尼美舒利是巴西消耗最多的药物之一，因此可以在废水中找到，因此在农业污水污泥中浓度为 ng L。本研究的目的是通过使用天然和人造土壤中的生物体进行毒性试验，采用其他抗炎药物对土壤中种子进行的研究浓度，评估抗炎药物尼美舒利的毒性。在这项工作中，在巴西圣保罗的天然热带土壤和热带人造土壤中评估了尼美舒利的毒性。无脊椎动物暴露于标称浓度为 200 的尼美舒利； 340； 578； 982 和 1670 mg 尼美舒利 kg 干土，按照 ISO 16.387 (2014) 标准进行繁殖试验，每个浓度重复 5 次。通过繁殖测试获得的结果是，对于天然土壤和人造土壤，所提供的每个浓度的 EC50 值分别为 699 毫克尼美舒利和 759 毫克尼美舒利公斤干燥土壤。然而，图基测试表明土壤之间没有显着差异。最后，这项研究表明，抗炎药尼美舒利在高浓度下对土壤动物的繁殖表现出毒理学作用。因此，强调识别和监测环境中这种新兴污染物的未知浓度的重要性。