The recent fast development of the microalgae-related biotechnologies has enabled the availability of sustainable and nutrients-rich raw materials to be used as substitution of conventional sources in aquafeed formulation. However the cost of energy of the photobioreactors used to produce these microorganisms still constrained their wider inclusion into aquafeed compounds. By exploiting industrial waste outputs, and were produced and utilised to formulate two experimental enrichments (LM1 and LM2). During a 60 days trial, their efficacy have been tested as metanauplii enrichments in the diet of European seabass () larvae, in comparison to a commercial control. Larvae were fed with isonitrogenous and isolipidic dietary treatments. Each dietary treatment presented a specific profile for the long chain polyunsaturated fatty acids ARA, DHA and EPA. Ratio of DHA/EPA in enriched of 4:1 as in the case of LM1 and LM2 in the presence of 1.4% of ARA (as a % on total fatty acid) guaranteed equal results to the control which displayed a ratio of DHA/EPA of 2:1 with 2.2% of ARA. No significant effects of the dietary treatments were detected in final survival, growth performance and incidence of skeletal anomalies. Regarding oxidative status, larvae fed with LM2 enrichment presented lower catalase activity than control larvae with no signs of oxidative damage, suggesting a potential antioxidant effect of LM2. The present study contributes to expanding the existing literature on successful utilisation of microalgae and cyanobacteria, used to produce valuable nutrients, in a perspective of circular nutrients economy.

中文翻译：

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从工业废物中产生的微藻和蓝藻混合物，用于富集卤虫：对欧洲鲈鱼（Dicentrarchus labrax）幼虫生长性能、抗氧化状态和异常率的影响

近年来，微藻相关生物技术的快速发展使得可持续且营养丰富的原材料能够在水产饲料配方中替代传统来源。然而，用于生产这些微生物的光生物反应器的能源成本仍然限制了它们更广泛地纳入水产饲料化合物中。通过开采工业废弃物产出物，分别生产和利用，配制出两种实验浓缩物（LM1和LM2）。在为期 60 天的试验中，与商业对照相比，它们的功效已通过欧洲鲈鱼 () 幼虫饮食中的后幼体富集进行了测试。幼虫用等氮和异脂饮食处理喂养。每种饮食治疗都呈现出长链多不饱和脂肪酸 ARA、DHA 和 EPA 的特定特征。富含 4:1 的 DHA/EPA 比例（如 LM1 和 LM2 中存在 1.4% ARA（占总脂肪酸的百分比））保证了与显示 DHA/EPA 比例的对照相同的结果2:1 与 2.2% ARA。饮食治疗对最终存活率、生长性能和骨骼异常发生率没有显着影响。关于氧化状态，喂食 LM2 富集物的幼虫表现出比对照幼虫更低的过氧化氢酶活性，且没有氧化损伤的迹象，表明 LM2 具有潜在的抗氧化作用。本研究有助于扩大现有的关于成功利用微藻和蓝细菌的文献，从循环营养经济的角度来看，这些微藻和蓝细菌可用于生产有价值的营养物质。